Dominant-negative inhibition of canonical Notch signaling in trophoblast cells does not disrupt placenta formation

Carrie J. Shawber, Dex Ann Brown-Grant, Tracy Wu, Jan K. Kitajewski, Nataki C. Douglas

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Proper development and function of the mammalian placenta requires interactions between embryo-derived trophoblasts and uterine endothelial cells to form mosaic vessels that facilitate blood flow to a developing conceptus. Notch signaling utilizes a cell-cell contact dependent mechanism to drive cell behaviors, such as differentiation and invasion. In mice, Notch2 is needed for proper placentation and embryo survival. We used transgenic mice with a dominant-negative form of Mastermind-like1 and Cyp19-Cre and Tpbpa-Cre drivers to inhibit canonical Notch signaling in trophoblasts. Both Cre drivers resulted in robust placental expression of dominantnegative Mastermind-like1. All pregnancies progressed beyond mid-gestation and morphological analyses of placentas revealed no differences between mutants and controls. Our data suggest that mouse placentation occurs normally despite dominant negative inhibition of trophoblast canonical Notch signaling and that Notch2 signaling via the canonical pathway is not necessary for placentation.

Original languageEnglish (US)
Article numberbio037721
JournalBiology Open
Issue number4
StatePublished - 2019

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)


  • Cyp19-Cre
  • Notch
  • Placenta
  • Tpbpa-Cre
  • Trophoblasts


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