Dopamine D1 receptor regulation of phospholipase C

P. A. Jose, P. Y. Yu, I. Yamaguchi, G. M. Eisner, M Maral Mouradian, C. C. Felder, R. A. Felder

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17 Scopus citations

Abstract

Dopamine is an endogenous catecholamine which exerts its actions by occupancy of specific receptors. Dopamine receptors are classified into two main groups: the two cloned D1-like receptors (D(1A) and D(1B) in rats; D(1B) is also known as D5 in humans) are linked to stimulation of adenylyl cyclase, while the three cloned D2-like receptors (D2 or D(2A), D3 or D(2B), D4 or D(2C)) are linked to inhibition of adenylyl cyclase. All these dopamine receptors originally cloned from the brain are expressed in tissues outside the central nervous system including the kidney. Dopamine regulates many cellular activities, including transmembrane ion transport. Activation of D1-like receptor decreases sodium transport by cAMP dependent and cAMP independent mechanisms. Dopamine, via D1-like receptors, may inhibit Na+/H+ exchange activity in renal brush border membranes by a cAMP independent/G(sα)-linked mechanism. Another cAMP independent pathway of sodium transport inhibition is mediated by phospholipase C, which has several isoforms (PLCβ, PLCγ, and PLCδ with several members in each). Catecholamines stimulate expression and activity of phospholipase C isoforms in a concentration, time, and receptor-dependent as well as regional and subcellular compartmental-specific manner. In renal cortical membranes, intrarenal administration of norepinephrine for 3-4 h increases PLCβ expression and activity but has no effect on PLCγ activity. In contrast, intrarenal administration of a D1 agonist for 3-4 h increases PLCβ1 but decreases PLCγ expression and activity. In membranes from LTK- cells transfected with the rat D(1A) receptor cDNA, D1 receptor stimulation initially increases and then in 4 h decreases PLCγ expression and activity, similar to the results found in renal cortical membranes. The initial increase of PLCγ expression and activity due to D1 receptors is mediated by protein kinase A.

Original languageEnglish (US)
Pages (from-to)S39-S42
JournalHypertension Research - Clinical and Experimental
Volume18
Issue numberSUPPL. 1
StatePublished - Jan 1 1995

All Science Journal Classification (ASJC) codes

  • Internal Medicine
  • Physiology
  • Cardiology and Cardiovascular Medicine

Keywords

  • Dopamine
  • Dopamine receptors
  • Ion transport
  • Phospholipase C

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    Jose, P. A., Yu, P. Y., Yamaguchi, I., Eisner, G. M., Mouradian, M. M., Felder, C. C., & Felder, R. A. (1995). Dopamine D1 receptor regulation of phospholipase C. Hypertension Research - Clinical and Experimental, 18(SUPPL. 1), S39-S42.