Abstract
The generation of dopamine (DA) neurons from stem cells holds great promise for future biomedical research and in the clinical treatment of neurodegenerative diseases, such as Parkinson's disease. Mesenchymal stem cells (MSCs) derived from the adult human bone marrow (BM) can be easily isolated and expanded in culture while maintaining their immense plasticity. Here, we describe a protocol to generate DA-producing cells from adult human MSCs using a cocktail that includes sonic hedgehog (SHH), fibroblast growth factor 8 (FGF8), and basic fibroblast growth factor (bFGF). Electrophysiological functional DA neurons could be achieved by further treatment with brain-derived neurotrophic factor (BDNF). In summary, a protocol is described for the induction of primary BM-derived human MSCs to specific transdifferentiation; in this case, functional DA neurons. The MSC-derived DA cells express DA-specific markers, synthesize, and secrete dopamine. The described method could be used to generate DA cells for various model systems in which DA-producing cells are implicated in pathophysiological conditions.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 295-303 |
| Number of pages | 9 |
| Journal | Methods in molecular biology (Clifton, N.J.) |
| Volume | 698 |
| DOIs | |
| State | Published - Jul 5 2011 |
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All Science Journal Classification (ASJC) codes
- Molecular Biology
- Genetics
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Dopaminergic neuronal differentiation protocol for human mesenchymal stem cells. / Trzaska, Katarzyna A.; Rameshwar, Pranela.
In: Methods in molecular biology (Clifton, N.J.), Vol. 698, 05.07.2011, p. 295-303.Research output: Contribution to journal › Article
TY - JOUR
T1 - Dopaminergic neuronal differentiation protocol for human mesenchymal stem cells.
AU - Trzaska, Katarzyna A.
AU - Rameshwar, Pranela
PY - 2011/7/5
Y1 - 2011/7/5
N2 - The generation of dopamine (DA) neurons from stem cells holds great promise for future biomedical research and in the clinical treatment of neurodegenerative diseases, such as Parkinson's disease. Mesenchymal stem cells (MSCs) derived from the adult human bone marrow (BM) can be easily isolated and expanded in culture while maintaining their immense plasticity. Here, we describe a protocol to generate DA-producing cells from adult human MSCs using a cocktail that includes sonic hedgehog (SHH), fibroblast growth factor 8 (FGF8), and basic fibroblast growth factor (bFGF). Electrophysiological functional DA neurons could be achieved by further treatment with brain-derived neurotrophic factor (BDNF). In summary, a protocol is described for the induction of primary BM-derived human MSCs to specific transdifferentiation; in this case, functional DA neurons. The MSC-derived DA cells express DA-specific markers, synthesize, and secrete dopamine. The described method could be used to generate DA cells for various model systems in which DA-producing cells are implicated in pathophysiological conditions.
AB - The generation of dopamine (DA) neurons from stem cells holds great promise for future biomedical research and in the clinical treatment of neurodegenerative diseases, such as Parkinson's disease. Mesenchymal stem cells (MSCs) derived from the adult human bone marrow (BM) can be easily isolated and expanded in culture while maintaining their immense plasticity. Here, we describe a protocol to generate DA-producing cells from adult human MSCs using a cocktail that includes sonic hedgehog (SHH), fibroblast growth factor 8 (FGF8), and basic fibroblast growth factor (bFGF). Electrophysiological functional DA neurons could be achieved by further treatment with brain-derived neurotrophic factor (BDNF). In summary, a protocol is described for the induction of primary BM-derived human MSCs to specific transdifferentiation; in this case, functional DA neurons. The MSC-derived DA cells express DA-specific markers, synthesize, and secrete dopamine. The described method could be used to generate DA cells for various model systems in which DA-producing cells are implicated in pathophysiological conditions.
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UR - http://www.scopus.com/inward/citedby.url?scp=79959810596&partnerID=8YFLogxK
U2 - 10.1007/978-1-60761-999-4_22
DO - 10.1007/978-1-60761-999-4_22
M3 - Article
C2 - 21431527
AN - SCOPUS:79959810596
VL - 698
SP - 295
EP - 303
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
SN - 1064-3745
ER -