Dosage compensation of the Drosophila white gene requires both the X chromosome environment and multiple intragenic elements

S. Qian, V. Pirrotta

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

The X-linked white genes when transposed to autosomes retains only partial dosage compensation. One copy of the gene in males expresses more than one copy but less than two copies in females. When inserted in ectopic X chromosome sites, the mini-white gene of the CaspeR vector can be fully dosage compensated and can even achieve hyperdosage compensation, meaning that one copy in males gives more expression than two copies in females. As sequences are removed gradually from the 5' end of the gene, we observe a progressive transition from hyperdosage compensation to full dosage compensation to partial dosage compensation. When the deletion reaches -17, the gene can no longer dosage compensate fully even on the X chromosome. A deletion reaching +173, 4 bp preceeding the AUG initiation codon, further reduces dosage compensation both on the X chromosome and on autosomes. This truncated gene can still partially dosage compensate on autosomes, indicating the presence of dosage compensation determinants in the protein coding region. We conclude that full dosage compensation requires an X chromosome environment and that the white gene contains multiple dosage-compensation determinants, some near the promoter and some in the coding region.

Original languageEnglish (US)
Pages (from-to)733-744
Number of pages12
JournalGenetics
Volume139
Issue number2
StatePublished - 1995
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics

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