Dose-response relationships between platelet activating factor and permeability--surface area product of FITC-dextran 150 in the hamster cheek pouch.

A. Y. Bekker, P. K. Dillon, J. Paul, A. B. Ritter, W. N. Durán

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Changes in vascular permeability to FITC-dextran 150 associated with topical application of platelet activating factor (PAF) in the hamster cheek pouch preparation were calculated using a mathematical model (Baxter et al., Microvasc. Res. 34:336, 1987). Least square estimates of apparent permeability-surface area product (PS) and interstitial diffusion coefficient (D) were calculated from the data and the mathematical model using a Marquart optimization algorithm. Calculated PS products varied with applied PAF concentration while the best-fit estimate of apparent D remained constant at 10.5 X 10(-10) cm2/s. Average baseline PS was 16.5 X 10(-8) ml/s. PS increased to 45.2 X 10(-8) ml/s and 52.3 X 10(-8) ml/s with application of 10(-8)M and 10(-7)M PAF, respectively. A further increase in PAF concentration to 10(-6)M decreased the calculated PS product to 30.5 X 10(-8) ml/s. PAF is a potential modulator of macromolecular permeability in the microcirculation. This is the first study which quantitates the changes in PS of FITC-dextran 150 as a function of PAF concentration.

Original languageEnglish (US)
Pages (from-to)433-446
Number of pages14
JournalMicrocirculation, endothelium, and lymphatics
Volume4
Issue number6
StatePublished - Dec 1988

All Science Journal Classification (ASJC) codes

  • Pharmacology

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