Double-humanized mouse model to study bone morphogenetic protein (BMP) signaling in tumor xenografts

Jenna Newman, Rachel NeMoyer, Andrew Zloza, John Langenfeld

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

The activation of the bone morphogenic protein (BMP) signaling pathway in cancer cells has been shown to enhance migration and tumor angiogenesis and promote survival. The BMP signaling pathway regulates benign cells in the tumor microenvironment and is a known regulator of immune cells. The development of BMP receptor inhibitors has allowed the study of tumor xenografts in mice. We describe a double-humanized mouse model with adoptively transferred human immune and human tumor cells that can be used to assess the effects of BMP inhibitors on these human cells in vivo.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages257-262
Number of pages6
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1891
ISSN (Print)1064-3745

Fingerprint

Bone Morphogenetic Proteins
Heterografts
Neoplasms
Bone Morphogenetic Protein Receptors
Tumor Microenvironment
Bone and Bones
Survival
Proteins

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Keywords

  • BMP inhibitor
  • Bone morphogenic protein
  • Cell culture
  • Double-humanized mouse model
  • ELISA
  • Flow cytometry
  • Immunohistochemistry
  • NOD scid gamma mice
  • Peripheral blood mononuclear cells
  • Western blotting
  • Xenografts

Cite this

Newman, J., NeMoyer, R., Zloza, A., & Langenfeld, J. (2019). Double-humanized mouse model to study bone morphogenetic protein (BMP) signaling in tumor xenografts. In Methods in Molecular Biology (pp. 257-262). (Methods in Molecular Biology; Vol. 1891). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8904-1_19
Newman, Jenna ; NeMoyer, Rachel ; Zloza, Andrew ; Langenfeld, John. / Double-humanized mouse model to study bone morphogenetic protein (BMP) signaling in tumor xenografts. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 257-262 (Methods in Molecular Biology).
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Newman, J, NeMoyer, R, Zloza, A & Langenfeld, J 2019, Double-humanized mouse model to study bone morphogenetic protein (BMP) signaling in tumor xenografts. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1891, Humana Press Inc., pp. 257-262. https://doi.org/10.1007/978-1-4939-8904-1_19

Double-humanized mouse model to study bone morphogenetic protein (BMP) signaling in tumor xenografts. / Newman, Jenna; NeMoyer, Rachel; Zloza, Andrew; Langenfeld, John.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 257-262 (Methods in Molecular Biology; Vol. 1891).

Research output: Chapter in Book/Report/Conference proceedingChapter

TY - CHAP

T1 - Double-humanized mouse model to study bone morphogenetic protein (BMP) signaling in tumor xenografts

AU - Newman, Jenna

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AU - Zloza, Andrew

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AB - The activation of the bone morphogenic protein (BMP) signaling pathway in cancer cells has been shown to enhance migration and tumor angiogenesis and promote survival. The BMP signaling pathway regulates benign cells in the tumor microenvironment and is a known regulator of immune cells. The development of BMP receptor inhibitors has allowed the study of tumor xenografts in mice. We describe a double-humanized mouse model with adoptively transferred human immune and human tumor cells that can be used to assess the effects of BMP inhibitors on these human cells in vivo.

KW - BMP inhibitor

KW - Bone morphogenic protein

KW - Cell culture

KW - Double-humanized mouse model

KW - ELISA

KW - Flow cytometry

KW - Immunohistochemistry

KW - NOD scid gamma mice

KW - Peripheral blood mononuclear cells

KW - Western blotting

KW - Xenografts

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T3 - Methods in Molecular Biology

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Newman J, NeMoyer R, Zloza A, Langenfeld J. Double-humanized mouse model to study bone morphogenetic protein (BMP) signaling in tumor xenografts. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 257-262. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-8904-1_19