Downregulation of endothelin-1 by interleukin-4 during gastric ulcer healing

Bronislaw L. Slomiany, Jerzy Piotrowski, Amalia Slomiany

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16 Scopus citations


We investigated the course of events associated with gastric ulcer healing by analyzing mucosal expression of interleukin-4 (IL-4), endothelin-1 (ET-1), tumor necrosis factor-α (TNF-α), and the activity of constitutive (cNOS) and inducible nitric oxide synthase (NOS-2). Ulcer onset was characterized by a massive epithelial apoptosis associated with a 5.7-fold increase in TNF-α, a 17.5-fold increase in NOS-2, and a 3.9-fold increase in ET-1, while mucosal expression of cNOS showed a 7.6-fold drop and IL-4 fell by 37.2%. Healing was accompanied by a rapid raise in IL-4; decrease in apoptosis, TNF-α, ET-1, and NOS-2; and a slow recovery in cNOS. The expression of IL-4 returned to control levels by the 7th day of healing and that of ET-1 and TNF-α by the 14th day, while apoptotic DNA fragmentation and the activity of NOS-2 remained significantly elevated beyond the 14-day period. The results suggest that a decrease in the mucosal level of IL-4 at ulcer onset may well be a key factor causing dysregulation of ET-1 production, induction of TNF-α, and triggering the apoptotic events that affect the efficiency of mucosal repair.

Original languageEnglish (US)
Pages (from-to)591-595
Number of pages5
JournalBiochemical and Biophysical Research Communications
Issue number2
StatePublished - Sep 24 1999

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


  • ET-1
  • Gastric ulcer
  • Healing
  • IL-4
  • NOS-2
  • TNF-α
  • cNOS


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