Droplet-based microfluidic platforms for single T cell secretion analysis of IL-10 cytokine

Tania Konry, Margarita Dominguez-Villar, Clare Baecher-Allan, David A. Hafler, Martin L. Yarmush

Research output: Contribution to journalArticle

66 Scopus citations

Abstract

Here we present a microfluidic method for the analysis of single cell secretions. The method co-encapsulates cells with microspheres conjugated with capture antibodies and detection fluorescence-labeled antibodies. The secreted substance captured on the microsphere surface and detected via detection antibodies generating a localized fluorescent signal on a microsphere surface. Using this method, CD4+CD25+ regulatory T cells were encapsulated and assayed to detect IL-10 secreting cell in population.

Original languageEnglish (US)
Pages (from-to)2707-2710
Number of pages4
JournalBiosensors and Bioelectronics
Volume26
Issue number5
DOIs
StatePublished - Jan 15 2011

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Keywords

  • Cell secretion
  • Droplets
  • Microfluidic
  • Microsphere
  • Single cell

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