TY - JOUR
T1 - Dual functional activity of semaphorin 3B is required for positioning the anterior commissure
AU - Julien, Falk
AU - Bechara, Ahmad
AU - Fiore, Roberto
AU - Nawabi, Homaira
AU - Zhou, Heather
AU - Hoyo-Becerra, Carolina
AU - Bozon, Muriel
AU - Rougon, Geneviève
AU - Grumet, Martin
AU - Püschel, Andreas W.
AU - Sanes, Joshua R.
AU - Castellani, Valérie
N1 - Funding Information:
We thank A. Chédotal, C. Faivre-Sarrailh, D. Karakogeos, M. Tessier-Lavigne, and A. Kolodkin for gifts of reagents. This work was supported by the CNRS ATIP program and the Fondation pour la Recherche Médicale (FRM) (V.C.), National Institute of Health (J.R.S.); grants from the DFG (A.W.P.), and a predoctoral fellowship (PFPI) from the Ministerio de Ciencia y Tecnologia, Spain (C.H.-B).
PY - 2005/10/6
Y1 - 2005/10/6
N2 - Chemorepulsion by semaphorins plays a critical role during the development of neuronal projections. Although semaphorin-induced chemoattraction has been reported in vitro, the contribution of this activity to axon pathfinding is still unclear. Using genetic and culture models, we provide evidence that both attraction and repulsion by Sema3B, a secreted semaphorin, are critical for the positioning of a major brain commissural projection, the anterior commissure (AC). NrCAM, an immunoglobulin superfamily adhesion molecule of the L1 subfamily, associates with neuropilin-2 and is a component of a receptor complex for Sema3B and Sema3F. Finally, we show that activation of the FAK/Src signaling cascade distinguishes Sema3B-mediated attractive from repulsive axonal responses of neurons forming the AC, revealing a mechanism underlying the dual activity of this guidance cue.
AB - Chemorepulsion by semaphorins plays a critical role during the development of neuronal projections. Although semaphorin-induced chemoattraction has been reported in vitro, the contribution of this activity to axon pathfinding is still unclear. Using genetic and culture models, we provide evidence that both attraction and repulsion by Sema3B, a secreted semaphorin, are critical for the positioning of a major brain commissural projection, the anterior commissure (AC). NrCAM, an immunoglobulin superfamily adhesion molecule of the L1 subfamily, associates with neuropilin-2 and is a component of a receptor complex for Sema3B and Sema3F. Finally, we show that activation of the FAK/Src signaling cascade distinguishes Sema3B-mediated attractive from repulsive axonal responses of neurons forming the AC, revealing a mechanism underlying the dual activity of this guidance cue.
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U2 - 10.1016/j.neuron.2005.08.033
DO - 10.1016/j.neuron.2005.08.033
M3 - Article
C2 - 16202709
AN - SCOPUS:25644450430
VL - 48
SP - 63
EP - 75
JO - Neuron
JF - Neuron
SN - 0896-6273
IS - 1
ER -