Dynamic profiling of hepatocyte stress responses in a microfluidic multi-clone living cell array

Kevin R. King, Sihong Wang, Daniel Irimia, Martin Yarmush, Arul Jayaraman, Mehmet Toner

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Hepatocytes in the liver utilize multiple signalling pathways to integrate extracellular stimuli and orchestrate appropriate cellular responses. Conventional experimental techniques are not well suited for studying dynamics or multiple pathways in parallel. Therefore, we have constructed a experimental platform for monitoring hepatocyte inflammatory signalling non-destructively in a multi-clone living cell reporter array. The microfluidic array allows seeding, culture, stimulation, and monitoring of key signaling pathways in the hepatocyte stress response using several stable GFP reporter clones. This integrated platform will allow us to decompose the effects of individual extracellular stimuli including cytokines, growth factors, hormones, and therpeutics to better appreciate coordinated regulation of the hepatocyte phenotype.

Original languageEnglish (US)
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference
Subtitle of host publication9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherTransducer Research Foundation
Pages533-535
Number of pages3
ISBN (Print)0974361119, 9780974361116
StatePublished - 2005
Externally publishedYes
Event9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States
Duration: Oct 9 2005Oct 13 2005

Publication series

NameMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Volume1

Other

Other9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
CountryUnited States
CityBoston, MA
Period10/9/0510/13/05

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Keywords

  • Cell culture
  • Cytokines
  • GFP reporters
  • Hepatocytes

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