Early and transient cytotoxic response of peritoneal cells from Fasciola hepatica-infected rats

Pierre Sibille, Omar Tliba, Chantal Boulard

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Experimental infection by F. hepatica was performed on rats. Early recruitment of the peritoneal cell population was observed and revealed transient parasite-killing activity, preceded and followed by a state of total unresponsiveness. The activation peaked at seven days post-infection (dpi) and was characterised by a massive peritoneal cell recruitment, a strong superoxide anion and nitric oxide (NO) production, that were coincident with the fasciolicide activity of these cells, as monitored by an in vitro decrease of juvenile fluke viability in a conditioned medium. The addition of L-NG-monomethyl arginine (LNMMA) to cell cultures abrogated both fasciolicide activity and NO production. Parasites started to die when NO production exceeded 25 μM and all juvenile flukes were killed by a 90 μM NO exposition (Lethal Dose 50 between 45.8 and 50.3 μM, 95% fiducial limits). However, when rat peritoneal cells were cultured in the presence of either infected or control rat serum, juvenile flukes were much more resistant to the oxidative burst, despite a massive attachment of rat peritoneal cells to the parasite tegument. These data suggest that a transient control of fasciolosis may take place in the peritoneum following the parasite intrusion but that the parasite efficiently scavenges the host cellular response to avoid destruction.

Original languageEnglish (US)
Pages (from-to)573-584
Number of pages12
JournalVeterinary Research
Volume35
Issue number5
DOIs
StatePublished - Sep 2004

All Science Journal Classification (ASJC) codes

  • veterinary(all)

Keywords

  • Cytotoxicity
  • F. hepatica
  • Nitric oxide
  • Rat peritoneal cells

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