Early presynaptic and late postsynaptic components contribute independently to brain-derived neurotrophic factor-induced synaptic plasticity

Janet Alder Suss, Smita Varia, Robert A. Crozier, Aisha Shaheen, Mark Plummer, Ira B. Black

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

Trophin-induced synaptic plasticity consists of both presynaptic and postsynaptic processes. The potential interdependence of these mechanisms and their temporal relationships are undefined. The synaptic vesicle protein Rab3A is required for the early, initial 10 min phase but not for the later phase of BDNF-enhanced transmission. We now examine the temporal distinction and mechanistic relationships between these phases of BDNF action. Rab3A mutant cells did not exhibit increased miniature EPSC frequency in response to BDNF in cell culture, indicating an absence of the presynaptic component. In contrast, BDNF enhanced postsynaptic glutamate-induced current in the mutant neurons as in the wild type, indicating that the postsynaptic component of the response was intact. Finally, the postsynaptic NMDA receptor subunit NR2B was phosphorylated at Tyr1472 by BDNF in Rab3A knock-outs, as shown previously in wild type. Our results are the first to demonstrate that presynaptic and postsynaptic components of BDNF-enhanced synaptic activity are independent and temporally distinct.

Original languageEnglish (US)
Pages (from-to)3080-3085
Number of pages6
JournalJournal of Neuroscience
Volume25
Issue number12
DOIs
StatePublished - Mar 23 2005

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Keywords

  • BDNF
  • Hippocampal
  • Postsynaptic
  • Presynaptic
  • Rab3A
  • Synaptic plasticity

Fingerprint Dive into the research topics of 'Early presynaptic and late postsynaptic components contribute independently to brain-derived neurotrophic factor-induced synaptic plasticity'. Together they form a unique fingerprint.

Cite this