Effect of a biofilm-degrading enzyme from an oral pathogen in transgenic tobacco on the pathogenicity of Pectobacterium carotovorum subsp. carotovorum

C. Ragunath, M. Shanmugam, M. Bendaoud, J. B. Kaplan, Narayanan Ramasubbu

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The hypothesis that dispersin B (DspB), an enzyme from the periodontal pathogen Aggregatibacter actinomycetemcomitans that degrades the extracellular matrix polysaccharide PGA, will inhibit biofilm formation of the soft rot pathogen Pectobacterium carotovorum subsp. carotovorum in infected plants was tested by constitutive expression of DspB in tobacco plants. All the transgenic plants expressed properly folded and active DspB enzyme, although at different expression levels. In virulence assays, even the transgenic plant line D10, which produced a low level of DspB compared to other lines, showed significant resistance against P. carotovorum subsp. carotovorum, suggesting that DspB could be a valuable agent for biological control of P. carotovorum subsp. carotovorum infection in crop plants.

Original languageEnglish (US)
Pages (from-to)346-354
Number of pages9
JournalPlant Pathology
Volume61
Issue number2
DOIs
StatePublished - Apr 1 2012

Fingerprint

Pectobacterium carotovorum
Pectobacterium carotovorum subsp. carotovorum
Biofilms
biofilm
Tobacco
Virulence
mouth
tobacco
pathogenicity
Genetically Modified Plants
genetically modified organisms
pathogens
transgenic plants
Actinobacillus actinomycetemcomitans
Enzymes
enzymes
Biological Control Agents
Prostaglandins A
Aggregatibacter actinomycetemcomitans
extracellular matrix

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science
  • Horticulture

Cite this

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abstract = "The hypothesis that dispersin B (DspB), an enzyme from the periodontal pathogen Aggregatibacter actinomycetemcomitans that degrades the extracellular matrix polysaccharide PGA, will inhibit biofilm formation of the soft rot pathogen Pectobacterium carotovorum subsp. carotovorum in infected plants was tested by constitutive expression of DspB in tobacco plants. All the transgenic plants expressed properly folded and active DspB enzyme, although at different expression levels. In virulence assays, even the transgenic plant line D10, which produced a low level of DspB compared to other lines, showed significant resistance against P. carotovorum subsp. carotovorum, suggesting that DspB could be a valuable agent for biological control of P. carotovorum subsp. carotovorum infection in crop plants.",
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Effect of a biofilm-degrading enzyme from an oral pathogen in transgenic tobacco on the pathogenicity of Pectobacterium carotovorum subsp. carotovorum. / Ragunath, C.; Shanmugam, M.; Bendaoud, M.; Kaplan, J. B.; Ramasubbu, Narayanan.

In: Plant Pathology, Vol. 61, No. 2, 01.04.2012, p. 346-354.

Research output: Contribution to journalArticle

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