Effect of bioactive growth surfaces on human mesenchymal stem cells: A pilot biomarker study to assess growth and differentiation

Liliana Craciun, Pranela Rameshwar, Steven J. Greco, Ted Deisenroth, Carmen Hendricks-Guy

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Mesenchymal stem cells (MSCs) are multipotent adult stem cells with the ability to differentiate into multiple cell lineages, and possess significant potential towards application in cell therapy and tissue engineering. A significant barrier to the effective implementation of human MSC (hMSC) therapies is the limited access to large quantities of viable, homogeneous cell populations produced in reproducible, consistent cultures. hMSCs are adherent cells whose morphology, as well as proliferation and differentiation potential, depend on the characteristics of the tissue culture surface they grow on. In the present study, we screened a panel of plastic surfaces possessing various physical characteristics for their ability to expand and maintain hMSCs in an undifferentiated state. The purpose of these studies was to identify materials which outperform conventional tissue culture polystyrene (TCPS). The plastic surfaces that were investigated were created by injection molding and were used either "as is" or after plasma treatment under an oxidative or reductive atmosphere. After 5 days culture, the effect of the growth surfaces on stem cell maintenance and differentiation was quantified by the expression of stem cell markers (OCT-4; NANOG; NOTCH1; PH-4; p21). Several surfaces exhibited an increase in the stem cell specific-and/or a decrease in the differentiation-specific genes, indicating a "positive result" compared to the TCPS reference standard. Of the many surface physical characteristics, the roughness and fibrous structure of a glass fiber-reinforced poly(styrene-acrylonitrile) polymeric surface had the most prevalent effect on facilitating hMSC expansion with preservation of stem cell function. These findings are not only significant in defining ideal conditions for hMSC growth in culture, but have broader implications for tissue engineering.

Original languageEnglish (US)
Title of host publicationThe Stem Cell Microenvironment and Its Role in Regenerative Medicine and Cancer Pathogenesis
PublisherRiver Publishers
Pages57-84
Number of pages28
ISBN (Electronic)9788793519008
ISBN (Print)9788793379930
StatePublished - Feb 1 2017

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Keywords

  • Bioactive surface
  • Cell differentiation
  • Cell growth
  • Human mesenchymal stem cells

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  • Cite this

    Craciun, L., Rameshwar, P., Greco, S. J., Deisenroth, T., & Hendricks-Guy, C. (2017). Effect of bioactive growth surfaces on human mesenchymal stem cells: A pilot biomarker study to assess growth and differentiation. In The Stem Cell Microenvironment and Its Role in Regenerative Medicine and Cancer Pathogenesis (pp. 57-84). River Publishers.