Effect of endotoxin on Erythrocyte Sedimentation Rate values

A. Shahzad, D. Kristol, R. R. Arora, C. R. Spillert

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Endotoxin (lipopolysaccharide, LPS) is part of the outer wall of a Gram-negative bacterial cell. LPS is a powerful mediator that causes a number of pathophysiological changes such as inflammations, tissue destruction, respiratory distress, capillary damage, intravascular coagulation and hypotension. The effect of LPS on Erythrocyte Sedimentation Rate (ESR) determinations has not previously been investigated. We performed in vitro studies by mixing human citrated whole blood (CWB) with LPS at a concentration 5 ug/mL. The samples (n=11) and (n=5) were incubated at 37°C for 10 minutes. The samples (n=11) were then refrigerated for 24 hours and incubated at 37°C for 30 minutes. The samples (n=5) were incubated for 24 hours at 37°C. The samples were allowed to settle for one hour and the ESR determined. The results indicate LPS treated blood had a significant reduction (p<0.05) in ESR values compared to the controls.

Original languageEnglish (US)
Title of host publicationProceedings of the IEEE 29th Annual Northeast Bioengineering Conference
EditorsStanley Reisman, Richard Foulds, Bruno Mantilla
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages256-257
Number of pages2
ISBN (Electronic)0780377672
DOIs
StatePublished - 2003
Event29th IEEE Annual Northeast Bioengineering Conference, NEBC 2003 - Newark, United States
Duration: Mar 22 2003Mar 23 2003

Publication series

NameProceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC
Volume2003-January
ISSN (Print)1071-121X
ISSN (Electronic)2160-7001

Other

Other29th IEEE Annual Northeast Bioengineering Conference, NEBC 2003
Country/TerritoryUnited States
CityNewark
Period3/22/033/23/03

All Science Journal Classification (ASJC) codes

  • Bioengineering

Keywords

  • Coagulation
  • Electric shock
  • In vitro
  • Microorganisms
  • Paramagnetic resonance
  • Plastics
  • Red blood cells
  • Refrigeration
  • Testing
  • White blood cells

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