Effect of entomopathogenic nematode concentration on survival during cryopreservation in liquid nitrogen

Cheng Bai, David I. Shapiro-Ilan, Randy Gaugler, Shuxia Yi

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Entomopathogenic nematodes are used for biological control of insect pests. A method for improved cryopreservation of infective juvenile stage nematodes has been developed using Steinernema carpocapsae and Heterorhabditis bacteriophora. Optimum survival for both species was achieved with 12,000 infective juveniles/ml in glycerol and 7,500/ml in Ringer's solution. For S. carpocapsae, maximum survival also was observed with 60,000 infective juveniles/ml in glycerol and 25,000/ml in Ringer's solution. These concentrations resulted in 100% post-cryopreservation survival of S. carpocapsae and 100% retention of original virulence to Galleria mellonella larvae. This is the first report of achieving 100% survival of an entomopathogenic nematode after preservation in liquid nitrogen. Maximum survival of H. bacteriophora following cryopreservation was 87%.

Original languageEnglish (US)
Pages (from-to)281-284
Number of pages4
JournalJournal of Nematology
Volume36
Issue number3
StatePublished - Sep 2004

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science

Keywords

  • Concentration
  • Cryobiology
  • Cryopreservation
  • Heterorhabditis bacteriophora
  • Steinernema carpocapsae
  • Survival

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