Effect of lipids and proteins on the viscosity of gastric mucus glycoprotein

V. L.N. Murty, J. Sarosiek, A. Slomiany, B. L. Slomiany

Research output: Contribution to journalArticlepeer-review

115 Scopus citations


The effect of associated lipids and covalently bound fatty acids, and the contribution of serum albumin and secretory IgA to the viscosity of dog gastric mucus glycoprotein was investigated. Using a cone/plate viscometer at shear rates between 1.15 - 230s-1, it was found that extraction of associated lipids from the glycoprotein lead to 80-85% decrease in the viscosity. Further loss (39%) in viscosity of the delipidated glycoprotein occurred following removal of covalently bound fatty acids. Reassociation of the delipidated glycoprotein with its neutral lipids increased the viscosity 3-fold, a 2.5-fold increase was obtained with glycolipids, and 2-fold with phospholipids. Preincubation of purified mucus glycoprotein with albumin or IgA resulted in the increase in viscosity. This increase in viscosity was proportional to albumin concentration up to 10%, and to IgA concentration up to 5%. The results show that interaction of lipids and proteins with mucus glycoprotein contributes significantly to the viscosity of gastric mucus.

Original languageEnglish (US)
Pages (from-to)521-529
Number of pages9
JournalBiochemical and Biophysical Research Communications
Issue number2
StatePublished - Jun 15 1984
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


Dive into the research topics of 'Effect of lipids and proteins on the viscosity of gastric mucus glycoprotein'. Together they form a unique fingerprint.

Cite this