Effect of locus coeruleus stimulation on regional cerebral oxygen consumption in the cat

E. Buchweitz, N. H. Edelman, H. R. Weiss

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Regional cerebral oxygen consumption was determined during stimulation of the intra-axial noradrenergic pathway to quantitate the metabolic effects of this manipulation on cerebral oxygen extraction, cerebral blood flow (CBF) and its regional distribution. Regional arterial and venous oxygen saturation were examined microspectrophotometrically. Regional CBF was examined using radioactively tagged microspheres (15 ± 3 μm in diameter). Oxygen consumption was calculated as the regional product of CBF and oxygen extraction. Bipolar concentric electrodes were stereotaxically implanted bilaterally in the locus coeruleus of α-chloralose anesthetized, artificially respired adult mongrel cats. The control group was killed after hemodynamic and CBF measurements were taken. The experimental group was sacrificed after these same measurements were taken before and during 10 min of bilateral locus coeruleus stimulation. The cats' heads were simultaneously sawed in 3 places and quickly frozen in liquid nitrogen-cooled propane. Systolic blood pressure was significantly increased during treatment. The heterogeneity of venous oxygen saturation was significantly reduced by stimulation. Average CBF and oxygen consumption were significantly decreased to 57% and 59% of control, respectively. Oxygen consumption was significantly reduced in the hypothalamus from 1.5 ± 0.3 to 0.9 ± 0.3 ml O2/min/100 g and from 3.5 ± 0.9 to 1.2 ± 0.4 ml O2/min/100 g in the cerebellum by treatment. Changes in the neuronal and/or synthetic cerebral activity produced regional decreases in cerebral oxygen consumption and secondarily altered CBF. These changes are probably due to interaction of the intraparenchymal noradrenergic pathways with other systems or processes in the brain. The direct innervation of cerebral arterioles and capillaries by the intracerebral noradrenergic pathway does not appear to play a significant role in the regulation of global or regional CBF during locus coeruleus stimulation, as evidenced by the unaltered average or regional oxygen supply to consumption ratio.

Original languageEnglish (US)
Pages (from-to)107-114
Number of pages8
JournalBrain research
Volume325
Issue number1-2
DOIs
StatePublished - Jan 28 1985

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Keywords

  • cat
  • cerebral O extraction
  • locus coeruleus stimulation
  • microspectrophotometric analysis
  • regional cerebral O consumption
  • regional cerebral blood flow

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