TY - JOUR
T1 - Effect of prostaglandins on tyrosylprotein sulfotransferase activity in rat submandibular salivary glands
AU - Kasinathan, C.
AU - Sundaram, R.
AU - William, S.
N1 - Funding Information:
Acknowledgements--This work was supportebdy UMDNJ Foundation, National Institutes of Health grant 2507RR06004-0a2n dN ationaIln stituteo f DentalR esearch grantD E09874-01.
PY - 1995/5
Y1 - 1995/5
N2 - 1. 1. Tyrosylprotein sulfotransferase (TPST) is a key enzyme in the processing of several secretory proteins, including those found in saliva. In this report, the effect of prostaglandins (PG) on TPST activity in submandibular salivary gland was investigated. 2. 2. The results revealed that PGE2 exhibited TPST stimulatory activity with a 1.5-fold stimulation at 100 μM concentration and a half maximal stimulation at 50 μM. The PGE2 stimulation was accompanied by an increase in the affinity of TPST towards sulfate acceptor (Km 1.4 μM → 0.12 μM) with little change in Vmax. 3. 3. The TPST activity was also stimulated by two other major prostaglandins of salivary glands, PGF2α and 6-Keto-PGF1α, however to lesser extent, 22 and 23%, respectively. Arachidonic acid, an intermediate prostaglandin precursor, had no effect on TPST activity. 4. 4. The results suggest that prostaglandins and in particular PGE2 may play a role in the regulation of TPST catalyzed secretory protein tyrosine sulfation in salivary glands.
AB - 1. 1. Tyrosylprotein sulfotransferase (TPST) is a key enzyme in the processing of several secretory proteins, including those found in saliva. In this report, the effect of prostaglandins (PG) on TPST activity in submandibular salivary gland was investigated. 2. 2. The results revealed that PGE2 exhibited TPST stimulatory activity with a 1.5-fold stimulation at 100 μM concentration and a half maximal stimulation at 50 μM. The PGE2 stimulation was accompanied by an increase in the affinity of TPST towards sulfate acceptor (Km 1.4 μM → 0.12 μM) with little change in Vmax. 3. 3. The TPST activity was also stimulated by two other major prostaglandins of salivary glands, PGF2α and 6-Keto-PGF1α, however to lesser extent, 22 and 23%, respectively. Arachidonic acid, an intermediate prostaglandin precursor, had no effect on TPST activity. 4. 4. The results suggest that prostaglandins and in particular PGE2 may play a role in the regulation of TPST catalyzed secretory protein tyrosine sulfation in salivary glands.
KW - Tyrosylprotein sulfotransferase
KW - prostaglandins
KW - salivary gland
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U2 - 10.1016/0306-3623(94)00224-B
DO - 10.1016/0306-3623(94)00224-B
M3 - Article
C2 - 7789731
AN - SCOPUS:0028945137
SN - 0306-3623
VL - 26
SP - 577
EP - 580
JO - General Pharmacology
JF - General Pharmacology
IS - 3
ER -