TY - JOUR
T1 - Effect of Saturated Very Long-Chain Fatty Acids on the Organization of Lipid Membranes
T2 - A Study Combining 2H NMR Spectroscopy and Molecular Dynamics Simulations
AU - Paz Ramos, Adrian
AU - Lagüe, Patrick
AU - Lamoureux, Guillaume
AU - Lafleur, Michel
N1 - Publisher Copyright:
© 2016 American Chemical Society.
PY - 2016/7/21
Y1 - 2016/7/21
N2 - Little is known about the interaction of very long-chain saturated fatty acids (VLCFAs) with biological membranes. However, this could play an important role on interleaflet interactions and signal transduction mechanisms in cells. The aim of this work is to determine how VLCFA structurally adapts in fluid phospholipid bilayers, since both species must exhibit a significant hydrophobic mismatch. The membrane organization has been described by means of 2H NMR and molecular dynamics simulations. Our results show that the protonation state affects the position and order of free fatty acids (FFAs) in phospholipid membranes. It was shown that the protonated FFA-C24 carboxyl group is located slightly under the POPC head group and therefore its acyl chain can interact with the lipids of the opposite leaflet. This interdigitation of the end of the acyl chain causes a second plateau observed in SC-D profiles, a very unusual feature in lipid systems.
AB - Little is known about the interaction of very long-chain saturated fatty acids (VLCFAs) with biological membranes. However, this could play an important role on interleaflet interactions and signal transduction mechanisms in cells. The aim of this work is to determine how VLCFA structurally adapts in fluid phospholipid bilayers, since both species must exhibit a significant hydrophobic mismatch. The membrane organization has been described by means of 2H NMR and molecular dynamics simulations. Our results show that the protonation state affects the position and order of free fatty acids (FFAs) in phospholipid membranes. It was shown that the protonated FFA-C24 carboxyl group is located slightly under the POPC head group and therefore its acyl chain can interact with the lipids of the opposite leaflet. This interdigitation of the end of the acyl chain causes a second plateau observed in SC-D profiles, a very unusual feature in lipid systems.
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U2 - 10.1021/acs.jpcb.6b04958
DO - 10.1021/acs.jpcb.6b04958
M3 - Article
C2 - 27351151
AN - SCOPUS:84979544708
SN - 1520-6106
VL - 120
SP - 6951
EP - 6960
JO - Journal of Physical Chemistry B
JF - Journal of Physical Chemistry B
IS - 28
ER -