Effect of snuff and nicotine on DNA methylation by 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone

Grazyna Prokopczyk, John D. Adams, Edmond J. Lavoie, Dietrich Hoffmann

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

In this study we assayed the effects of snuff and nicotine on the DNA methylation by 4-(methyhiitrosamino)-1-(3-pyridyl)-1-butanone (NNK), a powerful tobacco-specific N-nitrosa mine. Male F344 rats were pretreated for 2 weeks with either a solution of a snuff extract or 0.002% nicotine in the drinking water. Subsequently, the rats were given a single dose of NNK and the effects of snuff and nicotine on the methylation of guanine by NNK in the DNA of target organs of this carcinogenic nitrosamine were determined. Formation of 7-methylguanine in the liver, nasal mucosae and oral cavity and of O6-methaylguanine in the liver and oral cavity was much lower in the rats pretreated with snuff extract than in those not pretreated. On the other hand, pretreatment of the rats with nicotine had no significant effect on the methylation of DNA by NNK nor on the elimination constants of NNK and its major metabolite 4-(methylnitrosamino)-1-(3-pyridly)-1-butanol.

Original languageEnglish (US)
Pages (from-to)1395-1397
Number of pages3
JournalCarcinogenesis
Volume8
Issue number10
DOIs
StatePublished - Oct 1987
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Cancer Research

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