TY - JOUR
T1 - Effect of the anti-microtubule compound tubulozole on Leishmania protozoan parasites in vitro
AU - Fong, Dunne
N1 - Funding Information:
We thank Dr. Max Grogl for the gift of Leishmania major, Drs. Marion Man-Ying Chan and Robert Herman for helpful comments, Ms. Chiann-Chyi Chen for technical assistance, and Mrs. Lynn Weingart for manuscript preparation. This work was supported in part by grants CA 49359 from the National Institutes of Health, and the Charles and Johanna Busch Memorial Fund.
PY - 1993/2/15
Y1 - 1993/2/15
N2 - For the cis and trans stereoisomers of the synthetic anti-microtubule compound tubulozole, at micromolar concentrations, tubulozole-C is cytotoxic to mammalian cells whereas tubulozole-T is not. The effect of tubulozoles on the parasitic protozoan Leishmania was tested. For the promastigote stage of L. mexicana amazonensis, both isomers inhibited parasite growth. For the amastigote stage of L. mexicana amazonensis and L. major, within murine J774 macrophage line as host cells in vitro, tubulozole-T reduced the infective index. Despite the observation of macrophage cytotoxicity of tubulozole-T, this compound may be a potentially useful and novel anti-leishmanial drug.
AB - For the cis and trans stereoisomers of the synthetic anti-microtubule compound tubulozole, at micromolar concentrations, tubulozole-C is cytotoxic to mammalian cells whereas tubulozole-T is not. The effect of tubulozoles on the parasitic protozoan Leishmania was tested. For the promastigote stage of L. mexicana amazonensis, both isomers inhibited parasite growth. For the amastigote stage of L. mexicana amazonensis and L. major, within murine J774 macrophage line as host cells in vitro, tubulozole-T reduced the infective index. Despite the observation of macrophage cytotoxicity of tubulozole-T, this compound may be a potentially useful and novel anti-leishmanial drug.
KW - Leishmania major
KW - Leishmania mexicana amazonensis
KW - Microtubule
KW - Tubulin
KW - Tubulozole-T
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U2 - 10.1111/j.1574-6968.1993.tb06010.x
DO - 10.1111/j.1574-6968.1993.tb06010.x
M3 - Article
C2 - 8468004
AN - SCOPUS:0027502691
SN - 0378-1097
VL - 107
SP - 95
EP - 99
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 1
ER -