TY - JOUR
T1 - Effect of X-ray free-electron laser-induced shockwaves on haemoglobin microcrystals delivered in a liquid jet
AU - Grünbein, Marie Luise
AU - Gorel, Alexander
AU - Foucar, Lutz
AU - Carbajo, Sergio
AU - Colocho, William
AU - Gilevich, Sasha
AU - Hartmann, Elisabeth
AU - Hilpert, Mario
AU - Hunter, Mark
AU - Kloos, Marco
AU - Koglin, Jason E.
AU - Lane, Thomas J.
AU - Lewandowski, Jim
AU - Lutman, Alberto
AU - Nass, Karol
AU - Nass Kovacs, Gabriela
AU - Roome, Christopher M.
AU - Sheppard, John
AU - Shoeman, Robert L.
AU - Stricker, Miriam
AU - van Driel, Tim
AU - Vetter, Sharon
AU - Doak, R. Bruce
AU - Boutet, Sébastien
AU - Aquila, Andrew
AU - Decker, Franz Josef
AU - Barends, Thomas R.M.
AU - Stan, Claudiu Andrei
AU - Schlichting, Ilme
N1 - Funding Information:
The experiments were performed at the Linac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory. Use of the LCLS is supported by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences under Contract no. DE-AC02-76SF00515. The research was supported by the Max Planck Society and startup funds from Rutgers University Newark to C.A.S. Part of the sample injector used at LCLS for this research was funded by the National Institutes of Health, P41GM103393, formerly P41RR001209. We thank Yaroslav Aulin and Piotr Piotrowiak for assistance in testing the diode system.
Publisher Copyright:
© 2021, The Author(s).
PY - 2021/12/1
Y1 - 2021/12/1
N2 - X-ray free-electron lasers (XFELs) enable obtaining novel insights in structural biology. The recently available MHz repetition rate XFELs allow full data sets to be collected in shorter time and can also decrease sample consumption. However, the microsecond spacing of MHz XFEL pulses raises new challenges, including possible sample damage induced by shock waves that are launched by preceding pulses in the sample-carrying jet. We explored this matter with an X-ray-pump/X-ray-probe experiment employing haemoglobin microcrystals transported via a liquid jet into the XFEL beam. Diffraction data were collected using a shock-wave-free single-pulse scheme as well as the dual-pulse pump-probe scheme. The latter, relative to the former, reveals significant degradation of crystal hit rate, diffraction resolution and data quality. Crystal structures extracted from the two data sets also differ. Since our pump-probe attributes were chosen to emulate EuXFEL operation at its 4.5 MHz maximum pulse rate, this prompts concern about such data collection.
AB - X-ray free-electron lasers (XFELs) enable obtaining novel insights in structural biology. The recently available MHz repetition rate XFELs allow full data sets to be collected in shorter time and can also decrease sample consumption. However, the microsecond spacing of MHz XFEL pulses raises new challenges, including possible sample damage induced by shock waves that are launched by preceding pulses in the sample-carrying jet. We explored this matter with an X-ray-pump/X-ray-probe experiment employing haemoglobin microcrystals transported via a liquid jet into the XFEL beam. Diffraction data were collected using a shock-wave-free single-pulse scheme as well as the dual-pulse pump-probe scheme. The latter, relative to the former, reveals significant degradation of crystal hit rate, diffraction resolution and data quality. Crystal structures extracted from the two data sets also differ. Since our pump-probe attributes were chosen to emulate EuXFEL operation at its 4.5 MHz maximum pulse rate, this prompts concern about such data collection.
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U2 - 10.1038/s41467-021-21819-8
DO - 10.1038/s41467-021-21819-8
M3 - Article
C2 - 33723266
AN - SCOPUS:85102588958
SN - 2041-1723
VL - 12
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 1672
ER -