Effects of Moderate Pressure Overload Cardiac Hypertrophy on The Distribution of Creatine Kinase Isozymes

Myocardial activities and isozyme distributions of creatine kinase (CK) and lactate dehydrogenase (LDH) were measured in rats with moderate pressure overload hypertrophy. Three weeks after aortic banding, the ratio of left ventricular (LV) weight to body weight increased by 30%. Values for enzyme activity in the hyper-trophied LV were compared to values for control rats as well as to the contralateral relatively unaffected right ventricle (RV). In rats with moderate LV hypertrophy, total CK activity was unchanged. The percent MB-CK increased significantly (p<0.01) only in the hypertrophied LV, from 13 ± 1% to 19 ± 1% of total CK, while the sum of MM and mitochondrial-CK decreased from 86 ± 3 to 80 ± 3% (p<0.01). LDH activity increased (p<0.05) only in the hypertrophied ventricle from a control of 2.90 ± 0.13 to 3.21 ± 0.13 IU/mg protein, while the ratio of LDH activity at high to low substrate increased from 0.12 ± 0.02 to 0.14 ± 0.02 (p<0.05). Thus, the development of moderate pressure overload hypertrophy in the LV is associated with normal levels of total CK, but the percentage of MB-CK increases selectively in the primarily affected ventricle. Also, total LDH and LDH activity at high to low substrate concentration increases significantly in LV hypertrophy.