Effects of Probiotic Lactobacillus acidophilus Strain INMIA 9602 Er 317/402 and Putative Probiotic Lactobacilli on DNA Damages in the Small Intestine of Wistar Rats In Vivo

Astghik Z. Pepoyan, Marine H. Balayan, Lilit Malkhasyan, Anahit Manvelyan, Tatevik Bezhanyan, Ruzanna Paronikyan, Vardan V. Tsaturyan, Stepan Tatikyan, Shigeru Kamiya, Michael L. Chikindas

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Double-strand breaks in the DNA of the small intestine in male Wistar rats were studied using a neutral comet assay after 7 days of feeding with a single strain probiotic formulation Narine (Vitamax-E, Armenia), containing Lactobacillus acidophilus strain Er-2317/402 Narine, and putative probiotics L. rhamnosus Vahe and L. delbrueckii IAHAHI. Type 0 (undamaged DNA), type 1 (head diameter 13.18–17.08 μm), and type 2 (14.15-μm head diameter) damaged DNA comets were studied in control and lactobacilli-fed rats using the neutral comet assay. Lactobacilli-fed rats were shown to carry only type 0 (undamaged) DNA. Thus, the effects of probiotic Lactobacillus acidophilus strain INMIA 9602 Er 317/402 and putative probiotic lactobacilli on DNA damage in the small intestine of Wistar rats in vivo was shown, and the neutral comet assay is suggested as a potential tool for the in vivo selection of putative probiotics with DNA-protective activity.

Original languageEnglish (US)
Pages (from-to)905-909
Number of pages5
JournalProbiotics and Antimicrobial Proteins
Volume11
Issue number3
DOIs
StatePublished - Sep 15 2019

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Medicine
  • Molecular Biology

Keywords

  • Double-strand breaks of DNA
  • L. delbrueckii
  • L. rhamnosus
  • Neutral comet assay
  • Probiotic Narine
  • Small intestine

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