Effects of purified green and black tea polyphenols on cyclooxygenase- and lipoxygenase-dependent metabolism of arachidonic acid in human colon mucosa and colon tumor tissues

Jungil Hong, Theresa J. Smith, Chi Tang Ho, David A. August, Chung S. Yang

Research output: Contribution to journalArticlepeer-review

214 Scopus citations

Abstract

The effects of green and black tea polyphenols on cyclooxygenase (COX)- and lipoxygenase (LOX)-dependent arachidonic acid metabolism in normal human colon mucosa and colon cancers were investigated. At a concentration of 30 μg/mL, (-)-epigallocatechin-3-gallate (EGCG), (-)-epigallocatechin (EGC), and (-)-epicatechin-3-gallate (ECG) from green tea and theaflavins from black tea inhibited LOX-dependent activity by 30-75%. The formation of 5-, 12-, and 15-LOX metabolites was inhibited to a similar extent. Tea polyphenols also inhibited COX-dependent arachidonic acid metabolism in microsomes from normal colon mucosa, with ECG showing the strongest inhibition. The formation of thromboxane (TBX) and 12-hydroxyheptadecatrienoic acid (HHT) was decreased to a greater extent than other metabolites. The inhibitory effects of tea polyphenols on COX activity, however, were less pronounced in tumor microsomes than in normal colon mucosal microsomes. Theaflavins strongly inhibited the formation of zsTBX and HHT, but increased the production of prostaglandin E2 (PGE2) in tumor microsomes. The enhancing effect of theaflavins on PGE2 production was related to the COX-2 level in the microsomes. Although theaflavin inhibited ovine COX-2, its activity in the formation of PGE2 was stimulated by theaflavin when ovine COX-2 was mixed with microsomes, suggesting that theaflavin affects the interaction of COX-2 with other microsomal factors (e.g. PGE synthase). The present results indicate that tea polyphenols can affect arachidonic acid metabolism in human colon mucosa and colon tumors, and this action may alter the risk for colon cancer in humans.

Original languageEnglish (US)
Pages (from-to)1175-1183
Number of pages9
JournalBiochemical Pharmacology
Volume62
Issue number9
DOIs
StatePublished - Nov 1 2001

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Pharmacology

Keywords

  • Arachidonic acid
  • Colon
  • Cyclooxygenase
  • Lipoxygenase
  • Polyphenols
  • Tea

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