Effects of Stigmasterol and β-Sitosterol on Nonalcoholic Fatty Liver Disease in a Mouse Model: A Lipidomic Analysis

Simin Feng, Ling Gan, Chung S. Yang, Anna B. Liu, Wenyun Lu, Ping Shao, Zhuqing Dai, Peilong Sun, Zisheng Luo

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

To study the effects of stigmasterol and β-sitosterol on high-fat Western diet (HFWD)-induced nonalcoholic fatty liver disease (NAFLD), lipidomic analyses were conducted in liver samples collected after 33 weeks of the treatment. Principal component analysis showed these phytosterols were effective in protecting against HFWD-induced NAFLD. Orthogonal projections to latent structures-discriminate analysis (OPLS-DA) and S-plots showed that triacylglycerols (TGs), phosphatidylcholines, cholesteryl esters, diacylglycerols, and free fatty acids (FFAs) were the major lipid species contributing to these discriminations. The alleviation of NAFLD is mainly associated with decreases in hepatic cholesterol, TGs with polyunsaturated fatty acids, and alterations of free hepatic FFA. In conclusion, phytosterols, at a dose comparable to that suggested for humans by the FDA for the reduction of plasma cholesterol levels, are shown to protect against NAFLD in this long-term (33-week) study.

Original languageEnglish (US)
Pages (from-to)3417-3425
Number of pages9
JournalJournal of agricultural and food chemistry
Volume66
Issue number13
DOIs
StatePublished - Apr 4 2018

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Keywords

  • cholesterol
  • fatty liver
  • lipidomics
  • mice
  • stigmasterol
  • β-sitosterol

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