TY - JOUR
T1 - Effects of total glycosides from Baishouwu on human breast and prostate cancer cell proliferation
AU - Wang, Hongyan
AU - Wang, Qianben
AU - Srivastava, Rakesh K.
AU - Gong, Shu Sheng
AU - Lao, Lixing
AU - Fondell, Joseph D.
AU - Wang, Jiam Bei
PY - 2003/9
Y1 - 2003/9
N2 - Baishouwu (Cynanchum Auriculalum Royle ex Wight), a traditional Chinese herbal medicine, is used as a traditional anti-aging remedy in China. Previous studies suggested Baishouwu has multiple pharmacological actions. In this study, the total glycosides extracted from Baishouwu (CA/TG) were evaluated for its potential anti-tumor activity and mechanism on several human breast and prostate cancer cell lines. In [3H] thymidine incorporation assay, CA/TG inhibited the proliferation of cancer cells in a dose-dependent fashion. It also displayed anti-androgen activity on androgen sensitive cell line assessed by reporter gene assay. The morphological examination of CA/TG treated cells revealed overall shrinkage of nucleus, chromatin condensation and small nuclear fragments by DAPI staining, the characteristic pattern of DNA ladders were observed in cells exposed to CA/TG, indicating the involvement of apoptosis in its action. These results suggested that the CA/TG has preferred cytotoxicity against the cancer cells, and the action was due to its ability of preventing cell proliferation and inducing apoptosis.
AB - Baishouwu (Cynanchum Auriculalum Royle ex Wight), a traditional Chinese herbal medicine, is used as a traditional anti-aging remedy in China. Previous studies suggested Baishouwu has multiple pharmacological actions. In this study, the total glycosides extracted from Baishouwu (CA/TG) were evaluated for its potential anti-tumor activity and mechanism on several human breast and prostate cancer cell lines. In [3H] thymidine incorporation assay, CA/TG inhibited the proliferation of cancer cells in a dose-dependent fashion. It also displayed anti-androgen activity on androgen sensitive cell line assessed by reporter gene assay. The morphological examination of CA/TG treated cells revealed overall shrinkage of nucleus, chromatin condensation and small nuclear fragments by DAPI staining, the characteristic pattern of DNA ladders were observed in cells exposed to CA/TG, indicating the involvement of apoptosis in its action. These results suggested that the CA/TG has preferred cytotoxicity against the cancer cells, and the action was due to its ability of preventing cell proliferation and inducing apoptosis.
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UR - http://www.scopus.com/inward/citedby.url?scp=0141708045&partnerID=8YFLogxK
M3 - Article
C2 - 14526712
AN - SCOPUS:0141708045
VL - 9
SP - 62
EP - 66
JO - Alternative Therapies in Health and Medicine
JF - Alternative Therapies in Health and Medicine
SN - 1078-6791
IS - 5
ER -