EGR-1, a UV-inducible gene in p53-/- mouse cells

Weihua Zhang, Suzie Chen

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Changes in gene expression were examined in p53-/- and p53+/+ mouse cells after ultraviolet (UV) irradiation. Differential display was used to identify differentially expressed gene(s) in UV-treated p53-/- and p53+/+ cells. One of the differentially expressed genes was EGR-1 (early growth response gene-1), which was shown to be induced only in p53-/- cells. The induction of this gene by UV was detected as early as 0.5 h, peaked at 2 h, and returned to normal levels by 4 h. De novo protein synthesis was not required for UV-induced EGR-1 expression in p53-/- cells. Pretreatment of p53-/- cells with suramin, an inhibitor of growth factor receptors, completely suppressed UV-induced EGR-1 expression, suggesting that the induction may be mediated via the growth factor receptors. The presence of wild-type p53 suppressed the induction of EGR-1 after UV treatment. Overexpression of EGR-1 promoted the UV-induced transformation in p53+/+ cells, but not in p53-/- cells. These data suggested that EGR-1 may be an important player in the UV responses of mammalian cells and may influence UV-induced transformation.

Original languageEnglish (US)
Pages (from-to)21-30
Number of pages10
JournalExperimental cell research
Volume266
Issue number1
DOIs
StatePublished - May 15 2001

All Science Journal Classification (ASJC) codes

  • Cell Biology

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