Electrochemical determination of arylsulfatase activity using high-performance liquid chromatography

Rita T. Bradley, Paul Manowitz

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


A highly sensitive assay for arylsulfatase A was developed using high-performance liquid chromatography with electrochemical detection. The retention time of the enzymatic product, p-nitrocatechol, on a reverse phase column was approximately 2.0 min. The assay was able to detect 0.43 pmol p-nitrocatechol in a 20-μl ethanol extract of the reaction mixture. The coefficients of variation for seven determinations of the intra- and interassays were 9 and 8%, respectively. The levels of arylsulfatase A activity in human saliva samples and leukocyte and platelet lysates determined by this assay were within 6 and 11%, respectively, of the levels determined by a spectrophotometric assay. The high-performance liquid chromatography assay may have utility in measuring arylsulfatase A activity in biological samples with low activity or specific activity or in samples with compounds which interfere with the spectrophotometric assay.

Original languageEnglish (US)
Pages (from-to)33-38
Number of pages6
JournalAnalytical Biochemistry
Issue number1
StatePublished - Aug 15 1988

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


  • HPLC
  • arylsulfatase
  • electrochemical detection
  • hydrolases
  • proteins


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