TY - JOUR
T1 - Electron microscopy of cells infected with nonsense mutants of bacteriophage φ6
AU - Bamford, Dennis H.
AU - Mindich, Leonard
N1 - Funding Information:
We gratefully acknowledgeM s. Riikka Santalahti and Mr. J. Hagenzieker for assistance in the sectioning, and Dr. Claire Goldthwaite for the preparation of phages tocks.Our thanks also go to Mrs. Anna-be1H oward for preparation of the manuscript. This investigationw as supportedi n part by Public Health Service Grant AI-09861 (from the National Institute of Allergy and InfectiousD iseases,U SPHS), awarded to L.M.
PY - 1980
Y1 - 1980
N2 - Cells infected with nonsense mutants of bacteriophage φ6 were examined by electron microscopy. Mutants of class 812, which are incapable of synthesizing protein P8, the major component of the viral nucleocapsid, directed the formation of particles that appear to contain RNA and have the appearance of nucleocapsids in sectioned preparations. These particles are designated as filled procapsids. Mutants of class 12 or 9511, which are incapable of forming viral membrane, directed the synthesis of nucleocapsids. These particles are found distributed throughout the infected cells, although they appear to be somewhat aggregated when compared to the particles formed by the class 812 mutants. Mutants of class 511, which do not synthesize protein P5 or P11 which are involved in cell lysis, form particles indistinguishable from wild-type virus.
AB - Cells infected with nonsense mutants of bacteriophage φ6 were examined by electron microscopy. Mutants of class 812, which are incapable of synthesizing protein P8, the major component of the viral nucleocapsid, directed the formation of particles that appear to contain RNA and have the appearance of nucleocapsids in sectioned preparations. These particles are designated as filled procapsids. Mutants of class 12 or 9511, which are incapable of forming viral membrane, directed the synthesis of nucleocapsids. These particles are found distributed throughout the infected cells, although they appear to be somewhat aggregated when compared to the particles formed by the class 812 mutants. Mutants of class 511, which do not synthesize protein P5 or P11 which are involved in cell lysis, form particles indistinguishable from wild-type virus.
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U2 - 10.1016/0042-6822(80)90287-1
DO - 10.1016/0042-6822(80)90287-1
M3 - Article
C2 - 7445427
AN - SCOPUS:0019166535
SN - 0042-6822
VL - 107
SP - 222
EP - 228
JO - Virology
JF - Virology
IS - 1
ER -