Electronic Quantification of Protein Biomarkers Based on Bead Aggregate Sizing

Zhongtian Lin, Pengfei Xie, Xinnan Cao, Mehdi Javanmard

Research output: Contribution to journalArticlepeer-review

Abstract

We demonstrate a novel protein sensing method using detection of bead aggregates of different sizes in a micro-fluidic impedance cytometer. We utilize micro-fabricated coplanar electrodes at the base of our micro-channel to measure the impedance. When the single beads or bead aggregates pass through the electrodes, a difference in impedance change is detected. We measure these signals using a lock-in amplifier. Due to the difference in impedance change for unbound beads compared with protein-bound bead aggregates, we can differentiate the two types from each other electronically. We demonstrate proof-of-concept using streptavidin and biotin coated beads.

Original languageEnglish (US)
Article number7169504
Pages (from-to)6763-6764
Number of pages2
JournalIEEE Sensors Journal
Volume15
Issue number12
DOIs
StatePublished - Dec 2015

All Science Journal Classification (ASJC) codes

  • Instrumentation
  • Electrical and Electronic Engineering

Keywords

  • Cytometry
  • biosensing
  • microfluidics
  • protein detection

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