Electrophoresis using ultra-high voltages

Maribel Vazquez, Gareth McKinley, Luba Mitnik, Samantha Desmarais, Paul Matsudaira, Daniel Ehrlich

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Optimization of electrophoretic techniques is becoming an increasingly important area of research as microdevices are now routinely adapted for numerous biology and engineering applications. The present work seeks to optimize electrophoresis within microdevices by utilizing ultra-high voltages to increase sample concentration prior to separation. By imaging fluorescently-tagged DNA samples, the effects of both conventional and atypical voltage protocols on DNA migration and separation are readily observed. Experiments illustrate that short periods of high voltage during electrophoretic injection do not destroy the quality of DNA separations, and in fact can enhance sample concentration five-fold. This study presents data that illustrate increases in average resolution, and resolution of longer fragments, obtained from electrophoretic injections utilizing voltages between 85 and 850 V/cm.

Original languageEnglish (US)
Pages (from-to)163-171
Number of pages9
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume779
Issue number2
DOIs
StatePublished - Nov 5 2002
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

Keywords

  • DNA
  • Optimization
  • Ultra-high voltages electrophoresis

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