This report describes an initial investigation into electrophoretic elution and adsorption techniques which employ microporous membrane adsorbents. To probe the mechanism of elution, electrophoretic elution rates were measured as a function of key process parameters. The elution rate trends qualitatively obeyed the reaction-migration theory developed previously for packed column adsorbents. A quantitative theoretical description of the process is presented. Electrophoretic elution was observed to preserve the activity of a chemically-sensitive monoclonal antibody immunoadsorbent: when eluted using glycine buffer at pH 2.5, anti-bovine serum albumin immunomembranes lost 85% of their antigen binding capacities within three cycles, but essentially full binding capacity was retained over 15 cycles of electrophoretic elution. Electrophoretic adsorption is a technique whereby an electric field is used to facilitate adsorption. Compared with crossflow adsorption, electrophoretic adsorption increased antigen adsorption yields 10-fold (67% vs. 6.6%) while reducing adsorption times by a factor of eight (45 min vs. 6 hr). Adsorption data were interpreted using the numerical reaction-migration theory. Process scale-up and optimization strategies are discussed.
All Science Journal Classification (ASJC) codes
- Materials Science(all)
- Physical and Theoretical Chemistry
- Filtration and Separation
- affinity membranes
- electrophoretic elution