Electrophoretic injection within microdevices

Maribel Vazquez; Gareth McKinley; Luba Mitnik; Samantha Desmarais; Paul Matsudaira; Daniel Ehrlich

doi:10.1021/ac0110045

Electrophoretic injection within microdevices

Maribel Vazquez, Gareth McKinley, Luba Mitnik, Samantha Desmarais, Paul Matsudaira, Daniel Ehrlich

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

The flexibility of the microfabricated format creates unique opportunities for study of the electrophoretic process. The present work utilizes digital images to capture the motion of DNA samples during pre-electrophoretic processes. A systematic study of DNA loading and strong sample stacking (sample concentration effects) was performed in order to analyze realistic DNA analysis conditions within microdevices. Using digital imaging and microscopy, DNA sample profiles within the injector were analyzed by deconvolving the geometrical intensity profile into different velocity groups. This analysis illustrates the evolution of molecular separation into distinct migrating populations within the injector itself. The present study performed DNA injections within microfabricated devices imposing run voltages between 85 and 850 V/cm. Data from 3 different offset lengths of a double-T cross-injector, 10 different applied voltages, and 2 different sample preparation protocols are presented.

Original language	English (US)
Pages (from-to)	1952-1961
Number of pages	10
Journal	Analytical Chemistry
Volume	74
Issue number	9
DOIs	https://doi.org/10.1021/ac0110045
State	Published - May 1 2002
Externally published	Yes

All Science Journal Classification (ASJC) codes

Analytical Chemistry

Access to Document

10.1021/ac0110045

Cite this

@article{85e76d2628c94596b5e90916812e2010,

title = "Electrophoretic injection within microdevices",

abstract = "The flexibility of the microfabricated format creates unique opportunities for study of the electrophoretic process. The present work utilizes digital images to capture the motion of DNA samples during pre-electrophoretic processes. A systematic study of DNA loading and strong sample stacking (sample concentration effects) was performed in order to analyze realistic DNA analysis conditions within microdevices. Using digital imaging and microscopy, DNA sample profiles within the injector were analyzed by deconvolving the geometrical intensity profile into different velocity groups. This analysis illustrates the evolution of molecular separation into distinct migrating populations within the injector itself. The present study performed DNA injections within microfabricated devices imposing run voltages between 85 and 850 V/cm. Data from 3 different offset lengths of a double-T cross-injector, 10 different applied voltages, and 2 different sample preparation protocols are presented.",

author = "Maribel Vazquez and Gareth McKinley and Luba Mitnik and Samantha Desmarais and Paul Matsudaira and Daniel Ehrlich",

year = "2002",

month = may,

day = "1",

doi = "10.1021/ac0110045",

language = "English (US)",

volume = "74",

pages = "1952--1961",

journal = "Analytical Chemistry",

issn = "0003-2700",

number = "9",

}

TY - JOUR

T1 - Electrophoretic injection within microdevices

AU - Vazquez, Maribel

AU - McKinley, Gareth

AU - Mitnik, Luba

AU - Desmarais, Samantha

AU - Matsudaira, Paul

AU - Ehrlich, Daniel

PY - 2002/5/1

Y1 - 2002/5/1

N2 - The flexibility of the microfabricated format creates unique opportunities for study of the electrophoretic process. The present work utilizes digital images to capture the motion of DNA samples during pre-electrophoretic processes. A systematic study of DNA loading and strong sample stacking (sample concentration effects) was performed in order to analyze realistic DNA analysis conditions within microdevices. Using digital imaging and microscopy, DNA sample profiles within the injector were analyzed by deconvolving the geometrical intensity profile into different velocity groups. This analysis illustrates the evolution of molecular separation into distinct migrating populations within the injector itself. The present study performed DNA injections within microfabricated devices imposing run voltages between 85 and 850 V/cm. Data from 3 different offset lengths of a double-T cross-injector, 10 different applied voltages, and 2 different sample preparation protocols are presented.

AB - The flexibility of the microfabricated format creates unique opportunities for study of the electrophoretic process. The present work utilizes digital images to capture the motion of DNA samples during pre-electrophoretic processes. A systematic study of DNA loading and strong sample stacking (sample concentration effects) was performed in order to analyze realistic DNA analysis conditions within microdevices. Using digital imaging and microscopy, DNA sample profiles within the injector were analyzed by deconvolving the geometrical intensity profile into different velocity groups. This analysis illustrates the evolution of molecular separation into distinct migrating populations within the injector itself. The present study performed DNA injections within microfabricated devices imposing run voltages between 85 and 850 V/cm. Data from 3 different offset lengths of a double-T cross-injector, 10 different applied voltages, and 2 different sample preparation protocols are presented.

UR - http://www.scopus.com/inward/record.url?scp=0036558162&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036558162&partnerID=8YFLogxK

U2 - 10.1021/ac0110045

DO - 10.1021/ac0110045

M3 - Article

C2 - 12033291

AN - SCOPUS:0036558162

SN - 0003-2700

VL - 74

SP - 1952

EP - 1961

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 9

ER -

Electrophoretic injection within microdevices

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this