Eliminating primers from completed polymerase chain reactions with exonuclease VII

Honghua Li; Xiangfeng Cui; Norman Arnheim

doi:10.1093/nar/19.11.3139

Eliminating primers from completed polymerase chain reactions with exonuclease VII

Honghua Li, Xiangfeng Cui, Norman Arnheim

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Single-stranded oligonucleotide primers can be efficiently removed after PCR using E.coli exonuclease VII. Even only a few molecules of double stranded PCR prduct are unaffected by a treatment which eliminates 20 picomoles of primer in the presence of 500 ng of denatured genomic DNA. Exonuclease VII treatment is rapid and could simplify complicated multistep PCR protocols.

Original language	English (US)
Pages (from-to)	3139-3141
Number of pages	3
Journal	Nucleic acids research
Volume	19
Issue number	11
DOIs	https://doi.org/10.1093/nar/19.11.3139
State	Published - Jun 11 1991
Externally published	Yes

All Science Journal Classification (ASJC) codes

Genetics

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abstract = "Single-stranded oligonucleotide primers can be efficiently removed after PCR using E.coli exonuclease VII. Even only a few molecules of double stranded PCR prduct are unaffected by a treatment which eliminates 20 picomoles of primer in the presence of 500 ng of denatured genomic DNA. Exonuclease VII treatment is rapid and could simplify complicated multistep PCR protocols.",

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AB - Single-stranded oligonucleotide primers can be efficiently removed after PCR using E.coli exonuclease VII. Even only a few molecules of double stranded PCR prduct are unaffected by a treatment which eliminates 20 picomoles of primer in the presence of 500 ng of denatured genomic DNA. Exonuclease VII treatment is rapid and could simplify complicated multistep PCR protocols.

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