Eliminating primers from completed polymerase chain reactions with exonuclease VII

Honghua Li, Xiangfeng Cui, Norman Arnheim

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Single-stranded oligonucleotide primers can be efficiently removed after PCR using E.coli exonuclease VII. Even only a few molecules of double stranded PCR prduct are unaffected by a treatment which eliminates 20 picomoles of primer in the presence of 500 ng of denatured genomic DNA. Exonuclease VII treatment is rapid and could simplify complicated multistep PCR protocols.

Original languageEnglish (US)
Pages (from-to)3139-3141
Number of pages3
JournalNucleic acids research
Volume19
Issue number11
DOIs
StatePublished - Jun 11 1991
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics

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