TY - JOUR
T1 - Embryonic precursor cells from the rhombic lip are specified to a cerebellar granule neuron identity
AU - Alder, Janet
AU - Cho, Nam K.
AU - Hatten, Mary E.
N1 - Funding Information:
We are grateful to William Yang and Dr. Nathaniel Heintz for providing the RU49 cDNA and for extensive helpful discussions on these studies. Dr. Sally Temple carried out preliminary experiments critical to the results reported here. We thank Drs. Kathryn Zimmerman and Thomas Jessell for reading the manuscript. Mary Morrison, Magdalena Hofer, Dillon Patterson, and Christine Gallagher provided expert technical assistance. This work was supported by Program Project PHS NS30532-05 (M. E. H.) and The Cancer Research Fund of the Damon Runyon–Walter Winchell Foundation Fellowship, DRG-1342 (J. A.).
PY - 1996/9
Y1 - 1996/9
N2 - The specification of diverse classes of neurons is critical to the development of the cerebellar cortex. Here, we describe the purification of early embryonic precursors of cerebellar granule neurons from the rhombic lip, the dorsal aspect of the midbrain/hindbrain region. Isolation of rhombic lip cells reveals a homogenous population of precursor cells that express general neuronal markers and the granule cell marker RU49, but fail to extend neurites or express differentiation markers. Differentiation is induced by coculture with external germinal layer (EGL) cells, or their membranes, suggesting that a local inducing factor acts after formation of the EGL. Thus, proliferating precursors within the rhombic lip are specified to be granule cells very early, with the availability of an inducing factor increasing over the course of development.
AB - The specification of diverse classes of neurons is critical to the development of the cerebellar cortex. Here, we describe the purification of early embryonic precursors of cerebellar granule neurons from the rhombic lip, the dorsal aspect of the midbrain/hindbrain region. Isolation of rhombic lip cells reveals a homogenous population of precursor cells that express general neuronal markers and the granule cell marker RU49, but fail to extend neurites or express differentiation markers. Differentiation is induced by coculture with external germinal layer (EGL) cells, or their membranes, suggesting that a local inducing factor acts after formation of the EGL. Thus, proliferating precursors within the rhombic lip are specified to be granule cells very early, with the availability of an inducing factor increasing over the course of development.
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U2 - 10.1016/S0896-6273(00)80172-5
DO - 10.1016/S0896-6273(00)80172-5
M3 - Article
C2 - 8816703
AN - SCOPUS:0030248286
SN - 0896-6273
VL - 17
SP - 389
EP - 399
JO - Neuron
JF - Neuron
IS - 3
ER -