Embryonic stem cell-derived neuronally committed precursor cells with reduced teratoma formation after transplantation into the lesioned adult mouse brain

Marcel Dihné, Christian Bernreuther, Christian Hagel, Kai O. Wesche, Melitta Schachner

Research output: Contribution to journalArticlepeer-review

60 Scopus citations

Abstract

The therapeutic potential of embryonic stem (ES) cells in neurodegenerative disorders has been widely recognized, and methods are being developed to optimize culture conditions for enriching the cells of interest and to improve graft stability and safety after transplantation. Whereas teratoma formation rarely occurs in xenogeneic transplantation paradigms of ES cell-derived neural progeny, more than 70% of mice that received murine ES cell-derived neural precursor cells develop teratomas, thus posing a major safety problem for allogeneic and syngeneic transplantation paradigms. Here we introduce a new differentiation protocol based on the generation of substrate-adherent ES cell-derived neural aggregates (SENAs) that consist predominantly of neuronally committed precursor cells. Purified SENAs that were differentiated into immature but postmitotic neurons did not form tumors up to four months after syngeneic transplantation into the acutely degenerated striatum and showed robust survival.

Original languageEnglish (US)
Pages (from-to)1458-1466
Number of pages9
JournalSTEM CELLS
Volume24
Issue number6
DOIs
StatePublished - Jun 2006

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

Keywords

  • Cellular proliferation
  • Cellular therapy
  • Embryonic stem cells Committed progenitors
  • Neural differentiation
  • Stem cell transplantation

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