Empirical and computational design of iron-sulfur cluster proteins

Joanna Grzyb, Fei Xu, Vikas Nanda, Renata Łuczkowska, Eduard Reijerse, Wolfgang Lubitz, Dror Noy

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Here, we compare two approaches of protein design. A computational approach was used in the design of the coiled-coil iron-sulfur protein, CCIS, as a four helix bundle binding an iron-sulfur cluster within its hydrophobic core. An empirical approach was used for designing the redox-chain maquette, RCM as a four-helix bundle assembling iron-sulfur clusters within loops and one heme in the middle of its hydrophobic core. We demonstrate that both ways of design yielded the desired proteins in terms of secondary structure and cofactors assembly. Both approaches, however, still have much to improve in predicting conformational changes in the presence of bound cofactors, controlling oligomerization tendency and stabilizing the bound iron-sulfur clusters in the reduced state. Lessons from both ways of design and future directions of development are discussed. This article is part of a Special Issue entitled: Photosynthesis Research for Sustainability: from Natural to Artificial.

Original languageEnglish (US)
Pages (from-to)1256-1262
Number of pages7
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1817
Issue number8
DOIs
StatePublished - Aug 2012

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Cell Biology

Keywords

  • Computational design
  • Empirical design
  • Heme
  • Iron-sulfur cluster
  • Maquette
  • Protein design

Fingerprint

Dive into the research topics of 'Empirical and computational design of iron-sulfur cluster proteins'. Together they form a unique fingerprint.

Cite this