Endonuclease Activity Associated with the DNA · Protein Complex of Minicircular DNA of Escherichia coli 15

Joachim Messing, Walter L. Staudenbauer, Peter Hans Hofschneider

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The DNA · protein complex (form I*) of minicircular DNA of Escherichia coli has associated with it an endonucleolytic activity which converts the supercoiled form I into an open circular form II by introducing one single‐stranded break per molecule. This nick can occur with similar frequency in either strand. Prolonged incubation results in the formation of linear duplex molecules (form III). The endonuclease requires a divalent cation (Mg2+ or Mn2+) and is inhibited by high salt concentration and transfer RNA. It might provide a swivel mechanism during the replication of minicircular DNA.

Original languageEnglish (US)
Pages (from-to)39-44
Number of pages6
JournalEuropean Journal of Biochemistry
Volume36
Issue number1
DOIs
StatePublished - Jul 1973

All Science Journal Classification (ASJC) codes

  • Biochemistry

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