Engineering herbicide tolerance in transgenic plants

Dilip M. Shah, Robert B. Horsch, Harry J. Klee, Ganesh M. Kishore, Jill A. Winter, Nilgun E. Tumer, Cathy M. Hironaka, Patricia R. Sanders, Charles S. Gasser, Serdar Aykent, Ned R. Siegel, Stephen G. Rogers, Robert T. Fraley

Research output: Contribution to journalArticle

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Abstract

The herbicide glyphosate is a potent inhibitor of the enzyme 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase in higher plants. A complementary DNA (cDNA) done encoding EPSP synthase was isolated from a complementary DNA library of a glyphosate-tolerant Petunia hybrida cell line (MP4-G) that overproduces the enzyme. This cell line was shown to overproduce EPSP synthase messenger RNA as a result of a 20-fold amplification of the gene. A chimeric EPSP synthase gene was constructed with the use of the cauliflower mosaic virus 35S promoter to attain high level expression of EPSP synthase and introduced into petunia cells. Transformed petunia cells as well as regenerated transgenic plants were tolerant to glyphosate.

Original languageEnglish (US)
Pages (from-to)478-481
Number of pages4
JournalScience
Volume233
Issue number4762
DOIs
Publication statusPublished - Jan 1 1986
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • General

Cite this

Shah, D. M., Horsch, R. B., Klee, H. J., Kishore, G. M., Winter, J. A., Tumer, N. E., ... Fraley, R. T. (1986). Engineering herbicide tolerance in transgenic plants. Science, 233(4762), 478-481. https://doi.org/10.1126/science.233.4762.478