Engineering herbicide tolerance in transgenic plants

Dilip M. Shah, Robert B. Horsch, Harry J. Klee, Ganesh M. Kishore, Jill A. Winter, Nilgun Tumer, Cathy M. Hironaka, Patricia R. Sanders, Charles S. Gasser, Serdar Aykent, Ned R. Siegel, Stephen G. Rogers, Robert T. Fraley

Research output: Contribution to journalArticle

280 Citations (Scopus)

Abstract

The herbicide glyphosate is a potent inhibitor of the enzyme 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase in higher plants. A complementary DNA (cDNA) done encoding EPSP synthase was isolated from a complementary DNA library of a glyphosate-tolerant Petunia hybrida cell line (MP4-G) that overproduces the enzyme. This cell line was shown to overproduce EPSP synthase messenger RNA as a result of a 20-fold amplification of the gene. A chimeric EPSP synthase gene was constructed with the use of the cauliflower mosaic virus 35S promoter to attain high level expression of EPSP synthase and introduced into petunia cells. Transformed petunia cells as well as regenerated transgenic plants were tolerant to glyphosate.

Original languageEnglish (US)
Pages (from-to)478-481
Number of pages4
JournalScience
Volume233
Issue number4762
DOIs
StatePublished - Jan 1 1986

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herbicide resistance
transgenic plants
engineering
glyphosate
phosphates
Petunia
cell lines
gene amplification
complementary DNA
Petunia hybrida
Cauliflower mosaic virus
enzyme inhibitors
messenger RNA
cDNA libraries
herbicides
promoter regions
cells
enzymes
genes

All Science Journal Classification (ASJC) codes

  • General

Cite this

Shah, D. M., Horsch, R. B., Klee, H. J., Kishore, G. M., Winter, J. A., Tumer, N., ... Fraley, R. T. (1986). Engineering herbicide tolerance in transgenic plants. Science, 233(4762), 478-481. https://doi.org/10.1126/science.233.4762.478
Shah, Dilip M. ; Horsch, Robert B. ; Klee, Harry J. ; Kishore, Ganesh M. ; Winter, Jill A. ; Tumer, Nilgun ; Hironaka, Cathy M. ; Sanders, Patricia R. ; Gasser, Charles S. ; Aykent, Serdar ; Siegel, Ned R. ; Rogers, Stephen G. ; Fraley, Robert T. / Engineering herbicide tolerance in transgenic plants. In: Science. 1986 ; Vol. 233, No. 4762. pp. 478-481.
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abstract = "The herbicide glyphosate is a potent inhibitor of the enzyme 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase in higher plants. A complementary DNA (cDNA) done encoding EPSP synthase was isolated from a complementary DNA library of a glyphosate-tolerant Petunia hybrida cell line (MP4-G) that overproduces the enzyme. This cell line was shown to overproduce EPSP synthase messenger RNA as a result of a 20-fold amplification of the gene. A chimeric EPSP synthase gene was constructed with the use of the cauliflower mosaic virus 35S promoter to attain high level expression of EPSP synthase and introduced into petunia cells. Transformed petunia cells as well as regenerated transgenic plants were tolerant to glyphosate.",
author = "Shah, {Dilip M.} and Horsch, {Robert B.} and Klee, {Harry J.} and Kishore, {Ganesh M.} and Winter, {Jill A.} and Nilgun Tumer and Hironaka, {Cathy M.} and Sanders, {Patricia R.} and Gasser, {Charles S.} and Serdar Aykent and Siegel, {Ned R.} and Rogers, {Stephen G.} and Fraley, {Robert T.}",
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Shah, DM, Horsch, RB, Klee, HJ, Kishore, GM, Winter, JA, Tumer, N, Hironaka, CM, Sanders, PR, Gasser, CS, Aykent, S, Siegel, NR, Rogers, SG & Fraley, RT 1986, 'Engineering herbicide tolerance in transgenic plants', Science, vol. 233, no. 4762, pp. 478-481. https://doi.org/10.1126/science.233.4762.478

Engineering herbicide tolerance in transgenic plants. / Shah, Dilip M.; Horsch, Robert B.; Klee, Harry J.; Kishore, Ganesh M.; Winter, Jill A.; Tumer, Nilgun; Hironaka, Cathy M.; Sanders, Patricia R.; Gasser, Charles S.; Aykent, Serdar; Siegel, Ned R.; Rogers, Stephen G.; Fraley, Robert T.

In: Science, Vol. 233, No. 4762, 01.01.1986, p. 478-481.

Research output: Contribution to journalArticle

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AU - Horsch, Robert B.

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AU - Kishore, Ganesh M.

AU - Winter, Jill A.

AU - Tumer, Nilgun

AU - Hironaka, Cathy M.

AU - Sanders, Patricia R.

AU - Gasser, Charles S.

AU - Aykent, Serdar

AU - Siegel, Ned R.

AU - Rogers, Stephen G.

AU - Fraley, Robert T.

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Shah DM, Horsch RB, Klee HJ, Kishore GM, Winter JA, Tumer N et al. Engineering herbicide tolerance in transgenic plants. Science. 1986 Jan 1;233(4762):478-481. https://doi.org/10.1126/science.233.4762.478