Enhanced expression of dltabcd is associated with the development of daptomycin nonsusceptibuity in a clinical endocarditis isolate of staphylococcus aureus

Soo Jin Yang, Barry N. Kreiswirth, George Sakoulas, Michael R. Yeaman, Yan Q. Xiong, Ayumi Sawa, Arnold S. Bayer

Research output: Contribution to journalArticlepeer-review

96 Scopus citations

Abstract

Using isogenic clinical bloodstream Staphylococcus aureus strains from a patient with relapsing endocarditis, we investigated the transcriptional profiles of the mprF and dlt genes in the context of cell-surface charge and daptomycin nonsusceptibility. As in prior studies, a point mutation within mprF was observed in the daptomycin-nonsusceptible strain. However, neither the transcriptional profile of mprF nor the membrane phospholipid analyses were compatible with the anticipated mprF gain-in-function phenotype. In contrast, we demonstrated enhanced dit expression coincident with increased positive surface charge and reduced daptomycin binding.

Original languageEnglish (US)
Pages (from-to)1916-1920
Number of pages5
JournalJournal of Infectious Diseases
Volume200
Issue number12
DOIs
StatePublished - Dec 2009
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Infectious Diseases

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