Enhanced responses to mycobacterium tuberculosis antigens by human alveolar lymphocytes during active pulmonary tuberculosis

Stephan K. Schwander, Martha Torres, Eduardo Sada, Claudia Carranza, Esther Ramos, Magdalena Tary-Lehmann, Robert S. Wallis, Juan Sierra, Elizabeth A. Rich

Research output: Contribution to journalArticlepeer-review

93 Scopus citations

Abstract

Responses to mycobacterial and nonmycobacterial antigens were examined in bronchoalveolar cells (BAC) and peripheral blood mononuclear cells (PBMC) from patients with active pulmonary tuberculosis (n = 16) and healthy subjects (n = 23). DNA synthesis in BAC (but not PBMC) from tuberculosis patients was significantly increased in response to the mycobacterial antigens purified protein derivative (PPD), antigen 85, and mannose-capped lipoarabinomannan but not to nonmycobacterial antigens. The response to PPD was also increased in enriched alveolar lymphocytes from tuberculosis patients (P 05). The frequency of Interferon / but not interleukin-4- or -10-producing cells by ELISAspot was increased in PPD-stimulated BAC from patients with tuberculosis (P.05). Accessory function of alveolar macrophages for T lymphocyte responses was similar and suppressive activity was variably decreased in tuberculosis patients. Thus, there is compartmentalization of mycobacterial antigen-specific lymphocytes to the lungs during active tuberculosis that on challenge produce a Thl-type cytokine host response.

Original languageEnglish (US)
Pages (from-to)1434-1445
Number of pages12
JournalJournal of Infectious Diseases
Volume178
Issue number5
DOIs
StatePublished - Jan 1 1998
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General Medicine

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