Enhancement of 5-fluorouracil cytotoxicity on human colon cancer cells by retrovirus-mediated Interferon-α gene transfer

Hatem M.E. Sabaawy, Susumu Ikehara, Yasushi Adachi, Shou Quan, Eric Feldman, Ram Kancherla, Nader G. Abraham, Tauseef Ahmed

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Gene therapy has advantages in the treatment of a variety of disorders due to its selective expression within specific mammalian cells. Several reports documented the clinical effects of interferon-α (IFN-α) in management of patients with advanced colorectal carcinoma. We report for the first time, the successful transduction of human IFN-α gene into colon cancer cells, COLO 201 using a replication-defective retroviral vector. Retrovirus-containing supernatant from PA 317 packaging cells was used to infect colon cancer cells, COLO 201 and NIH 3T3 cells. Transient infection showed that cell proliferation and cell viability were significantly suppressed in colon cancer cells transduced with IFN-α gene. Moreover, IFN-α-transduced cells acquired less resistance to 5-FU induced apoptosis. These data demonstrate that IFN-α gene transfer may have a clinical application and can be combined with chemotherapy for treatment of advanced colorectal cancer.

Original languageEnglish (US)
Pages (from-to)1143-1151
Number of pages9
JournalInternational journal of oncology
Volume14
Issue number6
StatePublished - Jun 1999
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Keywords

  • 5-fluorouracil
  • Colon cancer
  • Gene transfer
  • Interferon-α

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