Enhancement of potato virus X synthesis in doubly infected tobacco occurs in doubly infected cells

Robert M. Goodman, A. Frank Ross

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

Enhancement of potato virus X (PVX) synthesis by potato virus Y (PVY) or tobacco mosaic virus (TMV) in tobacco is the result of increased synthesis per cell, not an increase in the number of cells infected. Ultrastructural features typical of PVX-infected cells and those typical of PVY-infected cells were present together in a majority of cells examined by electron microscopy from leaves supporting enhancement. PVX was detected in over 70% of protoplasts prepared from infected leaves and stained with fluorescein-labeled PVX antibody; the proportion was the same for cells from doubly infected and singly infected leaves. In leaves infected with TMV and PVX, 70% of the cells were doubly infected. The fluorescence intensity of individual cells after treatment with fluorescein-labeled PVX antibody, a measure of relative virus concentration, was greater in cells from leaves doubly infected by PVX and PVY than in cells from leaves infected by PVX alone.

Original languageEnglish (US)
Pages (from-to)16-24
Number of pages9
JournalVirology
Volume58
Issue number1
DOIs
StatePublished - Mar 1974
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Virology

Fingerprint

Dive into the research topics of 'Enhancement of potato virus X synthesis in doubly infected tobacco occurs in doubly infected cells'. Together they form a unique fingerprint.

Cite this