Enhancement of TPA-induced growth inhibition and apoptosis in myeloid leukemia cells by BAY 11-7082, an NF-κB inhibitor

Annette Hansson, Yarí E. Marín, Junghan Suh, Arnold B. Rabson, Suzie Chen, Eliezer Huberman, Richard L. Chang, Allan H. Conney, Xi Zheng

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

The phorbol ester, 12-O-tetradecanoylphorbol- 13-acetate (TPA) is a potent stimulator of differentiation and apoptosis in myeloid leukemia cells. In the present study, we investigated the role of the transcription factor NF-κB in TPA-induced growth inhibition and apoptosis in the myeloid leukemia HL-60 cell line and its TPA-resistant cell variant HL-525. Unlike the parental cell line, HL-525 cells are protein kinase C (PKC)-β deficient and resistant to TPA-induced differentiation and apoptosis. We found that treatment of HL-60 cells with TPA resulted in a concentration-dependent growth inhibition and an increase in apoptotic cells. TPA only had a small effect on growth and apoptosis in HL-525 cells. Treatment of HL-60 cells with TPA (0.64-3.2 nM) caused a rapid activation of NF-κB as determined by electrophoresis mobility shift assay (EMSA) and immunocytochemistry. Although the basal level of NF-κB activity was low in HL-60 cells, TPA-resistant HL-525 cells had a high basal level of NF-κB activity. Treatment of HL-525 cells with higher concentrations of TPA (16-80 nM) resulted in a further increase in N F - K B activity. (E)3-[(4-methylphenyl)-sulfony1]-2- propenenitrile (BAY 11-7082; BAY), which inhibits taBα phosphorylation and thus decreases NF-κB activation, was found to decrease TPA-induced nuclear translocation of NF-κB. Furthermore, BAY enhanced TPA-induced growth inhibition and apoptosis in both HL-60 and HL-525 cells. Results from the present study indicate that inhibition of NF-κB by BAY was associated with enhanced TPA-induced growth inhibition and apoptosis in human myeloid leukemia cells. TPA in combination with pharmacological inhibitors of NF-κB may improve the therapeutic efficacy of TPA and overcome the resistance to TPA in some myeloid leukemia patients.

Original languageEnglish (US)
Pages (from-to)941-948
Number of pages8
JournalInternational journal of oncology
Volume27
Issue number4
DOIs
StatePublished - Oct 2005

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Keywords

  • Apoptosis
  • Myeloid leukemia
  • NF-κB inhibitor

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