Enhancer, transcriptional, and cell fate plasticity precedes intestinal determination during endoderm development

Kushal K. Banerjee, Madhurima Saxena, Namit Kumar, Lei Chen, Alessiea Cavazza, Natalie H. Toke, Nicholas K. O’Neill, Shariq Madha, Unmesh Jadhav, Michael P. Verzi, Ramesh A. Shivdasani

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

After acquiring competence for selected cell fates, embryonic primordia may remain plastic for variable periods before tissue identity is irrevocably determined (commitment). We investigated the chromatin basis for these developmental milestones in mouse endoderm, a tissue with recognizable rostro–caudal patterning and transcription factor (TF)-dependent interim plasticity. Foregut-specific enhancers are as accessible and active in early midgut as in foregut endoderm, and intestinal enhancers and identity are established only after ectopic cis-regulatory elements are decommissioned. Depletion of the intestinal TF CDX2 before this cis element transition stabilizes foregut enhancers, reinforces ectopic transcriptional programs, and hence imposes foregut identities on the midgut. Later in development, as the window of chromatin plasticity elapses, CDX2 depletion weakens intestinal, without strengthening foregut, enhancers. Thus, midgut endoderm is primed for heterologous cell fates, and TFs act on a background of shifting chromatin access to determine intestinal at the expense of foregut identity. Similar principles likely govern other fate commitments.

Original languageEnglish (US)
Pages (from-to)1430-1442
Number of pages13
JournalGenes and Development
Volume32
Issue number21-22
DOIs
StatePublished - Nov 1 2018

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All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

Keywords

  • Chromatin plasticity
  • Developmental competence
  • Developmental plasticity
  • Fate determination
  • Homeodomain transcription factors
  • Lineage commitment
  • Tissue specification

Cite this

Banerjee, K. K., Saxena, M., Kumar, N., Chen, L., Cavazza, A., Toke, N. H., O’Neill, N. K., Madha, S., Jadhav, U., Verzi, M. P., & Shivdasani, R. A. (2018). Enhancer, transcriptional, and cell fate plasticity precedes intestinal determination during endoderm development. Genes and Development, 32(21-22), 1430-1442. https://doi.org/10.1101/gad.318832.118