Enhancing anthranilic acid biosynthesis using biosensor-assisted cell selection and in situ product removal

Zhenghong Li, Yingxi Lu, Xiaonan Wang, Ashil Vekaria, Ming Jiang, Haoran Zhang

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Anthranilic acid is an important chemical with recognized industrial values. As a precursor of amino acid tryptophan, anthranilic acid can be over-produced by engineering the tryptophan biosynthesis pathway. In this study, we utilized metabolic engineering approaches to enable anthranilic acid production in bacterium E. coli. Furthermore, an anthranilic acid biosensor-assisted cell selection technique was adopted to improve the microbial cell population composition and the overall production performance. An anthranilic acid in situ extraction method was also recruited to reduce the product accumulation and facilitate the cell selection. Based on these efforts, the engineered E. coli produced 688 mg/L anthranilic acid from glucose within 48 h. The results of this work hereby demonstrate the effectiveness of coupling biosensor-assisted cell selection and in situ product removal for microbial biosynthesis enhancement.

Original languageEnglish (US)
Article number107722
JournalBiochemical Engineering Journal
Volume162
DOIs
StatePublished - Oct 15 2020

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Environmental Engineering
  • Bioengineering
  • Biomedical Engineering

Keywords

  • Anthranilic acid
  • Biosensor-assisted selection
  • E. coli
  • In situ product removal

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