TY - JOUR
T1 - Enzymatic properties of bacteriophage t4 altered and modified rna polymerase as compared to dna-dependent rna polymerase from e. coli (ec 2.7.7.6)
AU - Schachner, Melitta
AU - Seifert, Wilfried
PY - 1971
Y1 - 1971
N2 - During infection with bacteriophage T4 the RNA polymerase of the E. coli host is changed in two consecutive steps: alteration and modification. It can be excluded that altered polymerase is a mixture of E. coli and modified polymerase. Alteration and modification manifest themselves enzymatically as a change of σ function. The basic catalytic properties of T4 altered, T4 modified and E. coli core-enzyme, as exemplified by sensitivity to inhibitors, heat inactivation, ionic strength dependence of kinetics, pH dependence and binding to double stranded T4-DNA, are not significantly different from each other.
AB - During infection with bacteriophage T4 the RNA polymerase of the E. coli host is changed in two consecutive steps: alteration and modification. It can be excluded that altered polymerase is a mixture of E. coli and modified polymerase. Alteration and modification manifest themselves enzymatically as a change of σ function. The basic catalytic properties of T4 altered, T4 modified and E. coli core-enzyme, as exemplified by sensitivity to inhibitors, heat inactivation, ionic strength dependence of kinetics, pH dependence and binding to double stranded T4-DNA, are not significantly different from each other.
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U2 - 10.1515/bchm2.1971.352.1.734
DO - 10.1515/bchm2.1971.352.1.734
M3 - Article
C2 - 4931577
AN - SCOPUS:0015060095
SN - 0018-4888
VL - 352
SP - 734
EP - 742
JO - Hoppe-Seyler's Zeitschrift fur Physiologische Chemie
JF - Hoppe-Seyler's Zeitschrift fur Physiologische Chemie
IS - 1
ER -