Enzymatic properties of bacteriophage t4 altered and modified rna polymerase as compared to dna-dependent rna polymerase from e. coli (ec 2.7.7.6)

Melitta Schachner, Wilfried Seifert

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

During infection with bacteriophage T4 the RNA polymerase of the E. coli host is changed in two consecutive steps: alteration and modification. It can be excluded that altered polymerase is a mixture of E. coli and modified polymerase. Alteration and modification manifest themselves enzymatically as a change of σ function. The basic catalytic properties of T4 altered, T4 modified and E. coli core-enzyme, as exemplified by sensitivity to inhibitors, heat inactivation, ionic strength dependence of kinetics, pH dependence and binding to double stranded T4-DNA, are not significantly different from each other.

Original languageEnglish (US)
Pages (from-to)734-742
Number of pages9
JournalHoppe-Seyler's Zeitschrift fur Physiologische Chemie
Volume352
Issue number1
DOIs
StatePublished - 1971

All Science Journal Classification (ASJC) codes

  • Biochemistry

Fingerprint

Dive into the research topics of 'Enzymatic properties of bacteriophage t4 altered and modified rna polymerase as compared to dna-dependent rna polymerase from e. coli (ec 2.7.7.6)'. Together they form a unique fingerprint.

Cite this