Enzymatic Sulfation of Glycosphingolipids in Rat Parotid Salivary Glands

E. Zdebska; Y. H. Liau; A. Slomiany; B. L. Slomiany

doi:10.1177/00220345830620100201

Enzymatic Sulfation of Glycosphingolipids in Rat Parotid Salivary Glands

E. Zdebska, Y. H. Liau, A. Slomiany, B. L. Slomiany

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The results of this study demonstrate that sulfatoglycosphingolipids of rat parotid salivary glands consist of galactosylceramide and lactosylceramide sulfates. The enzyme activity which catalyzes the formation of these compounds in vitro via transfer of sulfate from 3’-phosphoadenosine-5’-phosphosulfate to galactosyl- and lactosylceramide is located in the microsomal fraction of parotid glands. This sulfotransferase exhibits its optimum activity at pH 6.8 and requires the presence of Triton X-100, Mg2+, and F-.

Original language	English (US)
Pages (from-to)	1026-1030
Number of pages	5
Journal	Journal of dental research
Volume	62
Issue number	10
DOIs	https://doi.org/10.1177/00220345830620100201
State	Published - Oct 1983
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Dentistry

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title = "Enzymatic Sulfation of Glycosphingolipids in Rat Parotid Salivary Glands",

abstract = "The results of this study demonstrate that sulfatoglycosphingolipids of rat parotid salivary glands consist of galactosylceramide and lactosylceramide sulfates. The enzyme activity which catalyzes the formation of these compounds in vitro via transfer of sulfate from 3{\textquoteright}-phosphoadenosine-5{\textquoteright}-phosphosulfate to galactosyl- and lactosylceramide is located in the microsomal fraction of parotid glands. This sulfotransferase exhibits its optimum activity at pH 6.8 and requires the presence of Triton X-100, Mg2+, and F-.",

author = "E. Zdebska and Liau, {Y. H.} and A. Slomiany and Slomiany, {B. L.}",

year = "1983",

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AU - Zdebska, E.

AU - Liau, Y. H.

AU - Slomiany, A.

AU - Slomiany, B. L.

PY - 1983/10

Y1 - 1983/10

N2 - The results of this study demonstrate that sulfatoglycosphingolipids of rat parotid salivary glands consist of galactosylceramide and lactosylceramide sulfates. The enzyme activity which catalyzes the formation of these compounds in vitro via transfer of sulfate from 3’-phosphoadenosine-5’-phosphosulfate to galactosyl- and lactosylceramide is located in the microsomal fraction of parotid glands. This sulfotransferase exhibits its optimum activity at pH 6.8 and requires the presence of Triton X-100, Mg2+, and F-.

AB - The results of this study demonstrate that sulfatoglycosphingolipids of rat parotid salivary glands consist of galactosylceramide and lactosylceramide sulfates. The enzyme activity which catalyzes the formation of these compounds in vitro via transfer of sulfate from 3’-phosphoadenosine-5’-phosphosulfate to galactosyl- and lactosylceramide is located in the microsomal fraction of parotid glands. This sulfotransferase exhibits its optimum activity at pH 6.8 and requires the presence of Triton X-100, Mg2+, and F-.

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Enzymatic Sulfation of Glycosphingolipids in Rat Parotid Salivary Glands

Abstract

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